Skip to main content

Advertisement

SpringerLink
Log in

Effect of high-intensity irradiation from dental photopolymerization on the isolated and superfused vertebrate retina

  • Basic Science
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Background

Light or electromagnetic radiation may damage the neurosensory retina during irradiation of photopolymerizing resinous materials. Direct and indirect effects of irradiation emitted from polymerisation curing light may represent a severe risk factor for the eyes and the skin of the lamp operators, as well as for the patient’s oral mucosa.

Methods

Bovine superfused retinas were used to record their light-evoked electroretinogram (ERG) as ex vivo ERGs. Both the a- and the b-waves were used as indicators for retinal damage on the functional level. The isolated retinas were routinely superfused with a standard nutrient solution under normoglycemic conditions (5 mM D-glucose). The change in the a- and b-wave amplitude and implicit time, caused by low and high intensity irradiation, was calculated and followed over time.

Results

From the results, it can be deduced that the irradiation from LED high-power lamps affects severely the normal physiological function of the bovine retina. Irradiations of 1,200 lx irreversibly damaged the physiological response. In part, this may be reversible at lower intensities, but curing without using the appropriate filter will bleach the retinal rhodopsin to a large extent within 20 to 40 s of standard application times.

Conclusion

Constant exposure to intense ambient irradiation affects phototransduction (a-wave) as well as transretinal signalling. The proper use of the UV- and blue-light filtering device is highly recommended, and may prevent acute and long lasting damage of the neurosensory retina.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Youssef PN, Sheibani N, Albert DM (2011) Retinal light toxicity. Eye (Lond) 25:1–14

    Article  CAS  Google Scholar 

  2. Diffey BL (2002) Sources and measurement of ultraviolet radiation. Methods 28:4–13

    Article  PubMed  CAS  Google Scholar 

  3. Roberts JE (2011) Ultraviolet radiation as a risk factor for cataract and macular degeneration. Eye Contact Lens 37:246–249

    Article  PubMed  Google Scholar 

  4. Malik S, Rani S, Kumar V (2004) Wavelength dependency of light-induced effects on photoperiodic clock in the migratory blackheaded bunting (Emberiza melanocephala). Chronobiol Int 21:367–384

    Article  PubMed  Google Scholar 

  5. Chalam KV, Khetpal V, Rusovici R, Balaiya S (2011) A review: role of ultraviolet radiation in age-related macular degeneration. Eye Contact Lens 37:225–232

    Article  PubMed  CAS  Google Scholar 

  6. Organisciak DT, Vaughan DK (2010) Retinal light damage: mechanisms and protection. Prog Retin Eye Res 29:113–134

    Article  PubMed  Google Scholar 

  7. Bruzell Roll EM, Jacobsen N, Hensten-Pettersen A (2004) Health hazards associated with curing light in the dental clinic. Clin Oral Investig 8:113–117

    Article  PubMed  Google Scholar 

  8. Lüke M, Lüke C, Hescheler J, Schneider T, Sickel W (2005) Effects of phosphodiesterase type 5 inhibitor sildenafil on retinal function in isolated superfused retina. J Ocul Pharmacol Ther 21:305–314

    Article  PubMed  Google Scholar 

  9. Lüke M, Weiergräber M, Brand C, Siapich SA, Banat M, Hescheler J, Lüke C, Schneider T (2005) The isolated perfused bovine retina—a sensitive tool for pharmacological research on retinal function. Brain Res Brain Res Protocols 16:27–36

    Article  Google Scholar 

  10. Quinones-Hinojosa A, Malek JY, Ames A III, Ogilvy CS, Maynard KI (2003) Metabolic effects of hypothermia and its neuroprotective effects on the recovery of metabolic and electrophysiological function in the ischemic retina in vitro. Neurosurgery 52:1178–1186

    Article  PubMed  Google Scholar 

  11. Hanawa I, Tateishi T (1970) The effect of aspartate on the electroretinogram of the vertebrate retina. Experientia 26:1311–1312

    Article  PubMed  CAS  Google Scholar 

  12. Castelnuovo J, Tjan AH (1997) Temperature rise in pulpal chamber during fabrication of provisional resinous crowns. J Prosthet Dent 78:441–446

    Article  PubMed  CAS  Google Scholar 

  13. Baroudi K, Silikas N, Watts DC (2009) In vitro pulp chamber temperature rise from irradiation and exotherm of flowable composites. Int J Paediatr Dent 19:48–54

    Article  PubMed  Google Scholar 

  14. Karaarslan ES, Secilmis A, Bulbul M, Yildirim C, Usumez A (2011) Temperature increase beneath etched dentin discs during composite polymerization. Photomed Laser Surg 29:47–52

    Article  PubMed  CAS  Google Scholar 

  15. Onisor I, Asmussen E, Krejci I (2011) Temperature rise during photo-polymerization for onlay luting. Am J Dent 24:250–256

    PubMed  Google Scholar 

  16. Wu J, Seregard S, Algvere PV (2006) Photochemical damage of the retina. Surv Ophthalmol 51:461–481

    Article  PubMed  Google Scholar 

  17. Boulton M, Rozanowska M, Rozanowski B (2001) Retinal photodamage. J Photochem Photobiol B 64:144–161

    Article  PubMed  CAS  Google Scholar 

  18. Baumann C (1972) The regeneration and renewal of visual pigment in vertebrates. In: Dartnall HJA (ed) Handbook of sensory physiology. Springer, Berlin, Heidelberg, New York

    Google Scholar 

  19. Azuma K, Azuma M, Sickel W (1977) Regeneration of rhodopsin in frog rod outer segments. J Physiol 271:747–759

    PubMed  CAS  Google Scholar 

  20. Ewald A, Kühne W (1878) Untersuchungen über den Sehpurpur. II. Die Entstehung der Retinafarbe. Unters Physiol Inst Heidelb 1:248–290

    Google Scholar 

Download references

Acknowledgments

We thank Ms. Renate Clemens for her permanent technical assistance, and Mr. Maximilian Thelen as well as Ms. Valentina Safronjuk for help during retina preparations. The work was financially supported by the Köln Fortune Program/Faculty of Medicine, University of Köln (to TS).

Author information

Authors and Affiliations

  1. Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Koeln, Germany

    Mohammad Rassaei, Martin Thelen, Ramzi Abumuaileq, Jürgen Hescheler & Toni Schneider

  2. University Eye Hospital, University of Luebeck, Ratzeburger Allee 160, 23538, Luebeck, Germany

    Matthias Lüke

Authors
  1. Mohammad Rassaei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Martin Thelen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ramzi Abumuaileq
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jürgen Hescheler
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Matthias Lüke
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Toni Schneider
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Toni Schneider.

Additional information

Matthias Lüke and Toni Schneider shared senior authorship.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rassaei, M., Thelen, M., Abumuaileq, R. et al. Effect of high-intensity irradiation from dental photopolymerization on the isolated and superfused vertebrate retina. Graefes Arch Clin Exp Ophthalmol 251, 751–762 (2013). https://doi.org/10.1007/s00417-012-2235-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00417-012-2235-x

Keywords

Search

Navigation