Abstract
Purpose
We developed a new artificial image enhancement system and evaluated its usefulness in controlling intraoperative reflection and enhancing of Brilliant Blue G (BBG) staining.
Methods
The system was composed of three kinds of filters (a polarizing filter, a blue-enhancing filter, and a sharp-cut filter Y) and attached to the inferior surface of the operating microscope. Twenty-seven post-mortem extracted porcine eyes were used for a series of examinations. We performed surgery using the 23G-vitrectomy system with a halogen light and xenon lights and compared the reduction of intraoperative reflection under air condition and visibility and BBG contrast with and without this system. The evaluation of images was calculated in CIE 1976 (L*, a*, b*) color space (CIELAB) carried out by ImageJ software. The transmission of each filter and absorbance of BBG was measured by a spectrophotometer. We measured spectral irradiance at each wavelength about each filter from each light source with a spectroradiometer.
Results
Under both light sources, intraoperative reflection was controlled using a polarizing (PL) filter or combination of filters under air condition. Evaluation of the value of L* within the cutter surface was changed by 37.8 % under the halogen light, and 61.6 % (averaged) under the xenon light with inserted filters versus no filter. The BBG intensity difference was obtained with sharp-cut Y filter under both light source and PL with blue enhancing filter under the halogen light using each L*, a*, b* parameter with statistically significant (p < 0.01, 0.05). However, there was a relative decrease in the observation illuminance when the filter inserted according to the attenuation total spectral irradiance.
Conclusions
This system can reduce intraoperative reflections under the air condition and obtain an excellent BBG staining intensity induced by various light sources.
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Acknowledgments
This work was supported in part by a grant from the Japan Medical Association and grant-in-aid Health and Labour Sciences Research Grants of Research on Sensory Disorders from the Ministry of Health, Labour and Welfare, Tokyo, Japan. Grant title: Standardized protocol of early vitreous surgery for aggressive posterior retinopathy of prematurity.
Conflict of interest
Hiroshi Enaida is the developer of this system and the inventor of patent application (2011–15043 JP, 61/436,963US). Yoshiyuki Hachisuka is an employee of Hoya Corporation, which gave technical assistance, and is the inventor of patent application (2011–15043 JP, 61/436,963US). The remaining authors declare no conflicts of interest.
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Enaida, H., Hachisuka, Y., Yoshinaga, Y. et al. Development and preclinical evaluation of a new viewing filter system to control reflection and enhance dye staining during vitrectomy. Graefes Arch Clin Exp Ophthalmol 251, 441–451 (2013). https://doi.org/10.1007/s00417-012-2035-3
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DOI: https://doi.org/10.1007/s00417-012-2035-3