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Diagnostic yield of vitreous biopsy in presumed sarcoidosis-related posterior segment inflammation

  • Inflammatory Disorders
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

Diagnostic vitrectomy is frequently used to help establish a diagnosis in challenging uveitis cases. The usefulness of this procedure in patients with suspected sarcoidosis with posterior segment involvement, in whom a diagnosis cannot be determined by conventional methods, has not been well-described. We hypothesized that diagnostic vitrectomy would help establish a diagnosis of presumed sarcoidosis-related posterior segment inflammation in these challenging cases, and evaluated the diagnostic yield of vitreous biopsy in these eyes.

Design

Retrospective interventional case series.

Methods

Diagnostic vitrectomy was performed on eyes with intermediate, posterior, or panuveitis in which an etiology could not be characterized by history, clinical examination, ancillary testing, and/or laboratory testing. Retrospective chart review was conducted on consecutive eyes that underwent diagnostic, or diagnostic and therapeutic vitrectomy by a single surgeon between January 1989 and June 2006.

Results

Diagnostic and therapeutic vitrectomy was performed on 150 eyes. The final diagnosis was established by positive vitreous fluid analysis in 63 eyes (42 %). Eight of these vitreous specimens (5.3 %) yielded cytopathology consistent with presumed sarcoidosis-related posterior segment inflammation.

Conclusions

Sarcoidosis can cause characteristic inflammatory changes in the vitreous. In the appropriate clinical setting, cytopathologic assessment of these inflammatory changes observed in vitreous specimens can support the diagnosis of presumed sarcoidosis-related posterior segment inflammation, and help direct clinical management.

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Acknowledgements

This work received no external funding. The authors have no competing financial interests in this work. This study was approved by the Duke University Institutional Review Board, protocol ID #9351-07-1R0ER.

This trial has been registered with ClinicalTrials.gov. The registry number is NCT00609687.

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Authors and Affiliations

  1. Department of Ophthalmology, Albert Eye Research Institute, Duke University Eye Center, Duke University Medical Center, Durham, NC, USA

    Adrienne W. Scott, Prithvi Mruthyunjaya & Glenn J. Jaffe

  2. Department of Medicine, Division of Rheumatology and Clinical Immunology, Duke University Medical Center, Durham, NC, USA

    Rex M. McCallum

  3. Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Adrienne W. Scott

Authors
  1. Adrienne W. Scott
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  2. Prithvi Mruthyunjaya
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  3. Rex M. McCallum
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  4. Glenn J. Jaffe
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Correspondence to Adrienne W. Scott.

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Scott, A.W., Mruthyunjaya, P., McCallum, R.M. et al. Diagnostic yield of vitreous biopsy in presumed sarcoidosis-related posterior segment inflammation. Graefes Arch Clin Exp Ophthalmol 250, 1379–1385 (2012). https://doi.org/10.1007/s00417-012-1993-9

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  • DOI: https://doi.org/10.1007/s00417-012-1993-9

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