Abstract
The identification and quantification of metallic residues produced by gunshots, called gunshot residues (GSR), provide crucial elements in forensic investigations. The research has been largely focused on their collection onto the hands of suspected shooters, but the method is often burdened by risks of contamination. This research was focused on the possibility of sampling GSR trapped inside the nasal mucus of consenting shooters. Samples of the nasal mucus of “blank” control subjects and shooters were chemically analysed by Instrumental Neutron Activation Analysis (INAA), for residues of antimony (Sb) and barium (Ba), while lead (Pb) was excluded as ubiquitously environmental contaminant and due to high instrumental quantification limit (IQL) of INAA for this element. Shots were fired using two types of weapons (pistols and revolvers) and different firing sequences. The mucus was sampled at different times: immediately after the shots, after 30–60–120 and 180 min. Different amounts of Sb and Ba were detected between controls and shooters, witnessing the ability of the nasal mucus to retain GSR at concentrations significantly different even from the highest basal levels. Moreover, in order to simulate actual cases, nasal mucus from five groups of shooters was sampled after different shots with the same weapon and cartridges, immediately and after 1, 3, 12, and 24 h. The highest values were always found in the first 3 h from firing, for both weapons. Interestingly, for all the weapons, significant Sb and Ba concentrations were also found up to 12 h after firing, contrary to what occurs on hands, even though a progressive decrease was detected, with values below the detection threshold only after 24 h, thus demonstrating that GSR are persistent in nasal mucus. These first results proving that both Sb and Ba were qualitatively detectable in the nasal mucus of shooters indicate that the chemical analysis of the nasal mucus of suspected shooters may represent a promising tool in the forensic field since it is less burdened by problems related to sampling or contamination than the usual sampling on hand, providing that ammunitions employed contain Ba and Sb.
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References
Wolten GM, Nesbitt RS (1980) On the mechanism of gunshot residue particle formation. J Forensic Sci 25(3):533–545
Basu S (1982) Formation of gunshot residue. J Forensic Sci 27(1):72–91
Di Maio VJ (1998) Gunshot wounds: practical aspects of firearms, ballistics, and forensictechniques, second edition, Boca Raton (FL)
Schwoeble AJ, Exline DL (2000) Current methods in forensic gunshot residue analysis, 1st edn. CRC Press, Boca Raton
Dalby O, Butler D, Birkett W (2010) Analysis of gunshot residues and associated materials—a review. J Forensic Sci 55(4):924–943
Twibell ID, Home JM, Smalldon KW, Higgs DG, Hayes TS (1982) Assessment of solvent for the recovery of nitroglycerine from the hands using cotton swabs. J Forensic Sci 27(4):792–800
Basu S, Ferriss S (1980) A refined collection technique for rapid search of gunshot residues particles in the SEM, scan. Electron Microsc 1:375–384
Degaetano D, Siegel JA, Klomparens KL (1992) A comparison of 3 techniques developed for sampling and analysis of gunshot residues by scanning electron microscopy/energy dispersive X-ray analysis (SEM-EDX). J Forensic Sci 37(1):281–300
Romolo FS, Margot P (2001) Identification of gunshot residues: a critical review. Forensic Sci Int 119(2):195–211
Shaffer DK, Yi K (1992) A comparison of particle transfer efficiencies of two collection methods for the identification of gunshot residue on fabric surfaces using scanning electron microscopy-energy dispersive spectrometry. Scanning 21(2):99–100
Wrobel HA, Millar JJ, Kijek M (1998) Comparison of properties of adhesive tapes, tabs, and liquids used for the collection of gunshot residue and other materials for SEM analysis. J Forensic Sci 43(1):178–181
Capannesi G, Ciavola C, Sedda AF (1993) Determination of firing distance and firing angle by neutron activation analysis in a case involving gunshot wounds. Forensic Sci Int 61(2-3):75–84
Krishnan SS, Gillespie KA, Anderson EJ (1971) Rapid detection of firearm discharge residues by atomic absorption and neutron activation analysis. J Forensic Sci 16(2):144–151
Udey RN, Hunter BC, Smith RW (2011) Differentiation of bullet type based on the analysis of gunshot residue using inductively coupled plasma mass spectrometry. J Forensic Sci 56(5):1268–1276
Brazeau J, Wong RK (1997) Analysis of gunshot residues on human tissues and clothing by X-ray microfluorescence. J Forensic Sci 42(3):424–428
Brozek-Mucha Z, Jankowicz A (2001) Evaluation of the possibility of differentiation between various types of ammunition by means of GSR examination with SEM-EDX method. Forensic Sci Int 123(1):39–47
Brozek-Mucha Z, Zadora G (2003) Grouping of ammunition types by means of frequencies of occurrence of GSR. Forensic Sci Int 135(2):97–104
Hannigan TJ, McDermott SD, Greaney CM, O’Shaughnessy J, O’Brien CM (2015) Evaluation of gunshot residue (GSR) evidence: surveys of prevalence of GSR on clothing and frequency of residue types. Forensic Sci Int 257:177–181
Meng HH, Caddy B (1997) Gunshot residue analysis—a review. J Forensic Sci 42(4):553–570
Standard guide for gunshot residue analysis by scanning electron microscopy/energy-dispersive spectrometry, ASTM standards, designation E 1588-10e1. .
Rijnders MR, Stamouli A, Bolck A (2010) Comparison of GSR composition occurring at different locations around the firing position. J Forensic Sci 55(3):616–623
Schwartz RH, Zona CA (1995) A recovery method for airborne gunshot residue retained in human nasal mucus. J Forensic Sci 40(4):659–661
Honda A, Tsuji K, Matsuda Y, Hayashi T, Fukushima W, Sawahara T, Kudo H, Murayama R, Takano H (2015) Effects of air pollution-related heavy metals on the viability and inflammatory responses of human airway epithelial cells. Int J Toxicol 34(2):195–203
Sedda AF, Rossi G (2011) Bullets fragments identification by comparison of their chemical composition obtained using instrumental neutron activation analysis. Forensic Sci Int 206(1-3):e5–e7
Gibelli D, Brandone A, Andreola S, Porta D, Giudici E, Grandi MA, Cattaneo C (2010) Macroscopic, microscopic, and chemical assessment of gunshot lesions on decomposed pig skin. J Forensic Sci 55(4):1092–1097
Bhadkambekar CA, Swain KK, Mukherjee T, Sarin RK, Shukla SK, Kayasth S (2008) Zinc as a marker in viscera of suspected metal phosphide poisoning: a study by neutron activation analysis. J Anal Toxicol 32(9):760–762
Amadasi A, Gibelli D, Mazzarelli D, Porta D, Gaudio D, Salsarola D, Brandone A, Rizzi A, Cattaneo C (2015) Assets and pitfalls of chemical and microscopic analyses on gunshot residues in skeletonized bodies: a report of five cases. Int J Legal Med 129(4):819–824
Grima M, Hanson R, Tidy H (2014) An assessment of firework particle persistence on the hands and related police force practices in relation to GSR evidence. Forensic Sci Int 239:19–26
Tarifa A, Almirall JR (2015) Fast detection and characterization of organic and inorganic gunshot residues on the hands of suspects by CMV-GC-MS and LIBS. Sci Justice 55(3):168–175
Gallorini M (1995) Trace element monitoring in atmospheric pollution processes by neutron activation analysis. Microchem J 51(1-2):127–137
Gallorini M, Borroni PA, Bonardi M (1998) Trace elements in the atmospheric particulate of Milan and suburban areas: a study carried out by INAA. J Radioanal Nucl Chem 235(1-2):241–247
Gallorini M, Rizzio E, Birattari C, Bonardi M (1999) Content of trace elements in the respirable fractions of the air particulate of urban and rural areas monitored by neutron activation analysis. Biol Trace Elem Res 71–72(1):209–222
Bem H, Gallorini M, Rizzio E (2003) Comparative studies on the concentrations of some elements in the urban air particulate matter in Lodz City of Poland and in Milan, Italy. Environ Int 29(4):423–428
Brandone A, Riganti V (1981) Il contenuto di elementi in traccia nel pulviscolo dell’atmosfera urbana di Pavia. Rass Chim 2:59–62
Jalanti T, Henchoz P, Gallusser A, Bonfanti MS (1999) The persistence of gunshot residue on shooters’ hands. Sci Justice 39(1):48–52
Charles S, Geusens N (2011) A study of potential risk of gunshot residue transfer from special units of the police to arrested suspects. Forensic Sci Int 216(1-3):78–81
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Merli, D., Brandone, A., Amadasi, A. et al. The detection of gunshot residues in the nasal mucus of suspected shooters. Int J Legal Med 130, 1045–1052 (2016). https://doi.org/10.1007/s00414-016-1375-7
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DOI: https://doi.org/10.1007/s00414-016-1375-7