Abstract
The majority of STR loci are not ideal for the analysis of forensic samples with degraded and/or low template DNA. One alternative to overcome these limitations is the use of bi-allelic markers, which have low mutation rates and shorter amplicons. Human identification (HID) InDel marker panels have been described in several countries, including Brazil. The commercial kit available is, however, mostly suitable for Europeans, with lower discrimination power for other population groups. Recently, a combination of 49 InDel markers used in four different ethnic groups in the USA has been shown to be more informative than another panel from Portugal, already tested in a Rio de Janeiro sample. However, these 49 InDels have yet to be applied to other admixed or isolated populations. We assessed the efficiency of this panel in two urban admixed populations (Rio de Janeiro, Brazil; Tripoli, Libya) and one isolated Native Brazilian community. All markers are in Hardy-Weinberg equilibrium (HWE) after the Bonferroni correction, and no Linkage disequilibrium was detected. Assuming loci independence and no substructure effect, cumulative RMP was 2.7×10−18, 1.5×10−20, and 4.5×10−20 for Native Brazilian, Rio de Janeiro, and Tripoli populations, respectively. The overall Fst value was 0.05512. Rio de Janeiro and Tripoli showed similar admixture levels, however for Native Brazilians one parental cluster represented over 60 % of the total parental population. We conclude that this panel is suitable for HID on these urban populations, but is less efficient for the isolated group.
References
Butler JM, Shen Y, McCord BR (2003) The development of reduced size STR amplicons as tools for analysis of degraded DNA. J Forensic Sci 48:1054–1064
Kayser M, de Knijff P (2011) Improving human forensics through advances in genetics, genomics and molecular biology. Nat Publ Group 12:179–192. doi:10.1038/nrg2952
Sanchez JJ, Phillips C, Børsting C et al (2006) A multiplex assay with 52 single nucleotide polymorphisms for human identification. Electrophoresis 27:1713–1724. doi:10.1002/elps.200500671
Fridley BL, Biernacka JM (2011) Gene set analysis of SNP data: benefits, challenges, and future directions. Eur J Hum Genet 19:837–843. doi:10.1038/ejhg.2011.57
Budowle B, van Daal A (2008) Forensically relevant SNP classes. Biotechniques 44:603–8– 610. doi: 10.2144/000112806
LaRue BL, Ge J, King JL, Budowle B (2012) A validation study of the Qiagen Investigator DIPplex® kit; an INDEL-based assay for human identification. Int J Legal Med 126:533–540. doi:10.1007/s00414-012-0667-9
Friis SLE, Børsting C, Rockenbauer E, et al (2011) Typing of 30 insertion/deletions in Danes using the first commercial indelkit—Mentype. Forensic Sci Int Genet 1–3. doi: 10.1016/j.fsigen.2011.08.002
Neuvonen AM, Palo JU, Hedman M, Sajantila A (2012) Discrimination power of Investigator DIPplex loci in Finnish and Somali populations. Forensic Sci Int Genet 6:e99–e102. doi:10.1016/j.fsigen.2011.09.005
Torres SRR, Uehara CJS, Sutter-Latorre AF et al (2014) Population genetic data and forensic parameters of 30 autosomal InDel markers in Santa Catarina State population, Southern Brazil. Mol Biol Rep 41:5429–5433. doi:10.1007/s11033-014-3414-0
Pereira R, Phillips C, Alves C et al (2009) A new multiplex for human identification using insertion/deletion polymorphisms. Electrophoresis 30:3682–3690. doi:10.1002/elps.200900274
Manta F, Caiafa A, Pereira R et al (2012) Indel markers: genetic diversity of 38 polymorphisms in Brazilian populations and application in a paternity investigation with post mortem material. Forensic Sci Int Genet 6:658–661. doi:10.1016/j.fsigen.2011.12.008
Carvalho A, Pinheiro MF (2013) Population data of 30 insertion/delection polymorphisms from a sample taken in the North of Portugal. Int J Legal Med 127:65–67. doi:10.1007/s00414-012-0693-7
Pepinski W, Abreu-Glowacka M, Koralewska-Kordel M et al (2013) Population genetics of 30 INDELs in populations of Poland and Taiwan. Mol Biol Rep 40:4333–4338. doi:10.1007/s11033-013-2521-7
Liang W, Zaumsegel D, Rothschild MA et al (2013) Forensic Science International: Genetics Supplement Series. Forensic Sci Int: Genet Suppl Ser 4:e268–e269. doi:10.1016/j.fsigss.2013.10.137
Wang Z, Zhang S, Zhao S et al (2014) Population genetics of 30 insertion-deletion polymorphisms in two Chinese populations using Qiagen Investigator® DIPplex kit. Forensic Sci Int Genet 11:e12–e14. doi:10.1016/j.fsigen.2014.03.017
Kim EH, Lee HY, Yang IS et al (2014) Population data for 30 insertion-deletion markers in a Korean population. Int J Legal Med 128:51–52. doi:10.1007/s00414-013-0851-6
Seong KM, Park JH, Hyun YS et al (2014) Population genetics of insertion-deletion polymorphisms in South Koreans using Investigator DIPplex kit. Forensic Sci Int Genet 8:80–83. doi:10.1016/j.fsigen.2013.06.013
Akhteruzzaman S, Das SA, Hosen I, Ferdous A (2013) Forensic Science International: Genetics Supplement Series. Forensic Sci Int: Genet Suppl Ser 4:e348–e349. doi:10.1016/j.fsigss.2013.10.177
LaRue BL, Lagacé R, Chang C-W et al (2014) Characterization of 114 insertion/deletion (INDEL) polymorphisms, and selection for a global INDEL panel for human identification. Leg Med (Tokyo) 16(1):26–32. doi:10.1016/j.legalmed.2013.10.006
Aranda XG, Moura-Neto RS, Al-Deib AA et al (2010) Genetic data for D1S1677, D2S441, D4S2364, D10S1248, D14S1434 and D22S1045 miniSTR loci from Libya. Forensic Sci Int Genet 4:267–268. doi:10.1016/j.fsigen.2009.08.004
Aranda XG, Lage CAC, Planz JV, et al (2011) Genetic composition of six miniSTR in a Brazilian Mulatto sample population. J Forensic Leg Med 18:184–186. doi: 10.1016/j.jflm.2011.02.003
Purps J, Siegert S, Willuweit S et al (2014) A global analysis of Y-chromosomal haplotype diversity for 23 STR loci. Forensic Sci Int Genet 12:12–23. doi:10.1016/j.fsigen.2014.04.008
Budowle B, Smith J, Moretti T, DiZinno J (2000) DNA Typing Protocols: Molecular Biology and Forensic Analysis, 1st edn. Eaton Publishing Company, MA, US
Lewis P, Zaykin D (2001) Genetic Data Analysis: Computer program for the analysis of allelic data. Version 1.0 (d16c). http://lewis.eeb.uconn.edu/lewishome/software.html
Weir BS, cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 1358–1370
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959
Wei Y-L, Qin C-J, Dong H et al (2014) A validation study of a multiplex INDEL assay for forensic use in four Chinese populations. Forensic Sci Int Genet 9:e22–e25. doi:10.1016/j.fsigen.2013.09.002
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This work was supported in part by research grants from FAPERJ, CNPq, CAPES, and INMETRO.
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Moura-Neto, R.S., Silva, R., Mello, I.C. et al. Evaluation of a 49 InDel Marker HID panel in two specific populations of South America and one population of Northern Africa. Int J Legal Med 129, 245–249 (2015). https://doi.org/10.1007/s00414-014-1137-3
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DOI: https://doi.org/10.1007/s00414-014-1137-3