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Resveratrol mitigates genotoxicity induced by iodine-131 in primary human lymphocytes

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Abstract

The purpose of this study was to investigate the radioprotective effects of resveratrol as a natural product that protects against genotoxic actions of 131I in cultured human lymphocytes. Whole-blood samples from human volunteers were treated with resveratrol at doses of 0.5, 1, 5, and 50 μg/mL for 1 h, after which the lymphocytes were incubated with 131I (100 μCi/1.5 mL) for 2 h. The lymphocyte cultures were then mitogenically stimulated to enable evaluation of the number of micronuclei in cytokinesis-blocked binucleated cells. Incubation of lymphocytes with 131I induced genotoxicity, which was reflected by an increase in micronuclei frequency. At the doses tested, resveratrol significantly reduced micronuclei frequency. Maximal protective effects occurred at a dose of 1 μg/mL, with total micronuclei values being reduced by 65 % compared to controls. In conclusion, our results indicate protective effects of resveratrol at low doses against genetic damage and adverse effects induced by 131I administration.

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Acknowledgments

This research was the subject of a Pharm D thesis of Monireh Hedayati as a student of Mazandaran University of Medical Sciences. It was supported by Mazandaran University of Medical Sciences.

Conflict of interest

The authors declared no potential conflict of interest with respect to the research, authorship, and/or publication of this study.

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Authors and Affiliations

  1. Department of Radiopharmacy, Faculty of Pharmacy and Pharmaceutical Research Center, Mazandaran University of Medical Sciences, Sari, Iran

    Monireh Hedayati, Nayereh Shafaghati & Seyed Jalal Hosseinimehr

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  1. Monireh Hedayati
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  2. Nayereh Shafaghati
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  3. Seyed Jalal Hosseinimehr
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Correspondence to Seyed Jalal Hosseinimehr.

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Hedayati, M., Shafaghati, N. & Hosseinimehr, S.J. Resveratrol mitigates genotoxicity induced by iodine-131 in primary human lymphocytes. Radiat Environ Biophys 52, 287–291 (2013). https://doi.org/10.1007/s00411-013-0461-1

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  • DOI: https://doi.org/10.1007/s00411-013-0461-1

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