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Histopathological and immunological changes during the acute and recovery phase in Henoch–Schönlein purpura rabbit model

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A Letter to the Editor to this article was published on 14 May 2018

Abstract

Henoch–Schönlein purpura (HSP) is a systemic vasculitis mediated by autologous immune complex. Animal models of HSP are scarce. Here, we describe the characteristics of HSP rabbit model in the acute and recovery phase. First, we constructed the HSP rabbit models, and then assessed immunologic indicators of models by enzyme-linked immunosorbent assay and immunoturbidimetry. Histomorphological characteristics were analyzed by haematoxylin–eosin, immunofluorescence and special staining. In the acute stage (24 h) after antigen challenge, the model group rabbits featured skin ecchymosis and abnormal laboratory examination results. Three weeks following the allergic reaction, purple spots improved markedly, and edema and blood seeping decreased, but obvious inflammation was present in the skin, kidneys, joints, gastrointestinal, lung and liver. Serological results of CD4, CD/CD8, IL-2, IL-4, and TNF-α, IgA, IgG, TropI, Alb and T were still abnormal. IgA and C3 expressed in skin and kidney and eosinophils expressed in skin and lungs were increased. The rabbit model can mimic human HSP lesions in symptoms, pathology, and immunology and may provide valuable insight into the pathogenesis of HSP and serve as a tool for future therapeutic development targeting HSP.

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Abbreviations

HSP:

Henoch–Schönlein purpura

CD:

Cluster of differentiation

IL:

Interleukin

TNF:

Tumor necrosis factor

C:

Complement

Ig:

Immunoglobulin

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Acknowledgments

The work was supported by the Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Pathology Laboratory. The biochemical experiments were supported mainly by Beijing zhongtong Rambo clinical examination research institute.

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Authors and Affiliations

  1. Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, People’s Republic of China

    Yanhong Li, Xiaolong Sui, Hua Zhu, Yanfeng Xu, Lan Huang, Yuhuan Xu, Yunlin Han & Chuan Qin

  2. Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences, 5 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People’s Republic of China

    Yanhong Li, Xiaolong Sui, Hua Zhu, Yanfeng Xu, Lan Huang, Yuhuan Xu, Yunlin Han & Chuan Qin

  3. Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medical College, Beijing, People’s Republic of China

    Yanhong Li, Xiaolong Sui, Hua Zhu, Yanfeng Xu, Lan Huang, Yuhuan Xu, Yunlin Han & Chuan Qin

  4. Affiliated Hospital of Changchun College of Traditional Chinese Medicine, 1478 workers and Peasants Road, Chaoyang District, Changchun, 130021, People’s Republic of China

    Xiaochun Feng

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  1. Yanhong Li
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  2. Xiaolong Sui
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Correspondence to Xiaochun Feng or Chuan Qin.

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Li, Y., Sui, X., Zhu, H. et al. Histopathological and immunological changes during the acute and recovery phase in Henoch–Schönlein purpura rabbit model. Arch Dermatol Res 309, 21–30 (2017). https://doi.org/10.1007/s00403-016-1694-z

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  • DOI: https://doi.org/10.1007/s00403-016-1694-z

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