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Capparis spinosa protects against oxidative stress in systemic sclerosis dermal fibroblasts

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Abstract

High reactive oxygen species (ROS), Ha-Ras, and active ERK1/2 in fibroblasts play an essential role in the pathogenesis of systemic sclerosis (SSc). The present study was carried out to evaluate the effects of the ethanol extract from fruits of Capparis Spinosa L. (ECS) on oxidative stress and ROS-ERK1/2-Ha-Ras signal loop in SSc dermal fibroblasts in vitro. Cultured dermal fibroblasts from three SSc patients and three normal controls were treated with ECS by different concentration (10, 50, 100 μg/ml). ECS significantly reduced the production of O2 –, H2O2, and ROS in SSc fibroblasts in a dose-dependent manner. ECS effectively minimized the loss of cell viability and apoptosis induced by H2O2 in normal and SSc fibroblasts. Furthermore, the protective effect of ECS on SSc fibroblasts was more significant than on normal ones. ECS decreased the expression of P-ERK1/2 and Ha-Ras in a dose-dependent manner. In conclusion, ECS exhibits a notable activity in protecting against oxidative stress and interrupting of ROS-ERK1/2-Ha-Ras signal loop in SSc, suggesting its potential protective effects against skin sclerosis.

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Abbreviations

SSc:

Systemic sclerosis

ECS:

The ethanol extract from fruits of Capparis Spinosa L.

ROS:

Reactive oxygen species

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

ERK:

Extracellular signal-regulated kinase

DCFH-DA:

Fluorescent probe 2′-7′-Dichlorodihydrofluorescin diacetate

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Acknowledgments

This work was supported by Provincial Natural Science Foundation of Zhejiang Province (Y2080357), and the Science and Technology Foundation of Zhejiang Province Project (2009C33135).

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Authors and Affiliations

  1. Department of Dermatology, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, 310009, Hangzhou, China

    Yue-lan Cao & Min Zheng

  2. Department of STD and AIDS Prevention, Hangzhou Center for Disease Control and Prevention, 310006, Hangzhou, China

    Xin Li

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  1. Yue-lan Cao
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  2. Xin Li
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Correspondence to Yue-lan Cao.

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Cao, Yl., Li, X. & Zheng, M. Capparis spinosa protects against oxidative stress in systemic sclerosis dermal fibroblasts. Arch Dermatol Res 302, 349–355 (2010). https://doi.org/10.1007/s00403-009-0998-7

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  • DOI: https://doi.org/10.1007/s00403-009-0998-7

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