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The effect of hydroxyapatite particle size on viscoelastic properties and calcium release from a thermosensitive triblock copolymer

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Abstract

Well-defined “smart” injectable hydrogel based on hydrophilic poly(ethylene glycol) (PEG) and hydrophobic poly(lactic acid-co-glycolic acid) (PLA/PGA) copolymer (PLGA-PEG-PLGA) gelling at the body temperature was modified by bioactive hydroxyapatite (HAp) in the form of micro-, nano-, and core-shell particles (μ-HAp, n-HAp, and CS-x, respectively) to be applicable as calcium delivery system in bone regeneration. Viscoelastic moduli increased with HAp content as expected. Whereas systems containing μ-HAp or CS-x particles maintained two sol-gel and gel-sol phase transitions, the n-HAp containing system showed only one sol-gel phase transition due to the strong interactions between polymer chain and the n-HAp surface. In vitro, studies proved the controlled uniform release of calcium cations from both CS-x and n-HAp over the 9-day period without any initial burst release.

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Acknowledgements

This research was carried out under the project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II and FP7 project no. 60436 named “BioScaffolds”. We acknowledge the CF Cryo-electron Microscopy and Tomography supported by the CIISB research infrastructure (LM2015043 funded by MEYS CR) for their support with obtaining scientific data presented in this paper.

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Authors and Affiliations

  1. CEITEC - Brno University of Technology, 123, Purkynova, Czech Republic

    I. Chamradová, L. Vojtová, K. Částková & J. Jančář

  2. SCITEG, a.s, Brno, Czech Republic

    L. Vojtová & J. Jančář

  3. Material Research Centre, Brno University of Technology, Brno, Czech Republic

    P. Diviš

  4. Institute of Materials Chemistry, Brno University of Technology, Brno, Czech Republic

    P. Diviš & J. Jančář

  5. CEITEC - Masaryk University, Brno, Czech Republic

    M. Peterek

Authors
  1. I. Chamradová
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  2. L. Vojtová
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  3. K. Částková
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  4. P. Diviš
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  5. M. Peterek
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  6. J. Jančář
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Corresponding author

Correspondence to I. Chamradová.

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Statement of disclosure

This study was funded by CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II (grant number LQ1601) and MEYS CR (grant number LM2015043).

Authors Ivana Chamradova, Lucy Vojtová, Josef Jančář, and Pavel Diviš have received research grants from CEITEC 2020 (grant number LQ1601). Author Miroslav Peterek has received research grant from MEYS CR (grant number LM2015043) and Klára Částková FP7 project No. 60436 named “BioScaffolds”.

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The authors declare that they have no conflict of interest.

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Chamradová, I., Vojtová, L., Částková, K. et al. The effect of hydroxyapatite particle size on viscoelastic properties and calcium release from a thermosensitive triblock copolymer. Colloid Polym Sci 295, 107–115 (2017). https://doi.org/10.1007/s00396-016-3983-7

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  • DOI: https://doi.org/10.1007/s00396-016-3983-7

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