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The formation of mixed micelles of sugar surfactants and phospholipids and their interactions with hyaluronan

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Abstract

The aggregation of sugar surfactants and the incorporation of phospholipids into sugar surfactant micelles were investigated by means of fluorescence spectroscopy. Two representatives from the family of alkyl glucosides were studied: dodecyl-β-D-maltoside and octyl-β-D-glucopyranoside. The presence of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) in the sugar surfactant system promoted the formation of new structures at sugar surfactant concentration below its critical micelle concentrations. According to the aggregation number measurements studied by a fluorescence quenching of hexadecylpyridinium chloride measurements, the premicellar aggregates formed from octyl-β-D-glucopyranoside and DPPC were composed of the third of molecules compared to the octyl-β-D-glucopyranoside micelles. Interactions of the formed mixed micelle systems composed of sugar surfactant and DPPC with hyaluronan were explored. The addition of hyaluronan had different effects on the dodecyl-β-D-maltoside/DPPC and octyl-β-D-glucopyranoside/DPPC mixed systems. In addition, the mixed system of dodecyl-β-D-maltoside and lecithin was studied, but possibly, only a coexistence of lipid aggregates and sugar surfactant micelles was observed.

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Abbreviations

DM:

Dodecyl-β-D-maltoside

OG:

Octyl-β-D-glucopyranoside

EmPI:

Emission polarity index

ExPI:

Excitation polarity index

CMC:

Critical micelle concentrations

CPC:

Hexadecylpyridinium chloride monohydrate

DPPC:

1,2-Dipalmitoyl-sn-glycero-3-phosphatidylcholine

Hya:

Hyaluronan

CBA:

Concentration at the beginning of an aggregation

R 2 :

Reliability

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Acknowledgments

This work was supported by Project Nr. LO1211, Materials Research Centre at FCH BUT-Sustainability and Development (National Program for Sustainability I, Ministry of Education, Youth and Sports of the Czech Republic).

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Authors and Affiliations

  1. Institute of Physical and Applied Chemistry, Faculty of Chemistry, Brno University of Technology, Purkyňova 118/464, 612 00, Brno, Czech Republic

    Jana Burdíková, Filip Mravec & Miloslav Pekař

  2. Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118/464, 612 00, Brno, Czech Republic

    Jana Burdíková, Filip Mravec & Miloslav Pekař

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  1. Jana Burdíková
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  2. Filip Mravec
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Correspondence to Filip Mravec.

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This study was funded by Project Nr. LO1211, Materials Research Centre at FCH BUT-Sustainability and Development (National Program for Sustainability I, Ministry of Education, Youth and Sports of the Czech Republic).

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Burdíková, J., Mravec, F. & Pekař, M. The formation of mixed micelles of sugar surfactants and phospholipids and their interactions with hyaluronan. Colloid Polym Sci 294, 823–831 (2016). https://doi.org/10.1007/s00396-016-3840-8

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