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N,N,N-trimethylchitosan iodide complexes with a weak or a strong polyacid and nanoparticles thereof

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Abstract

Polyelectrolyte complexes (PECs) were prepared from N,N,N-trimethylchitosan iodide (TMCh) of different molar mass and a weak polyacid-poly(acrylic acid) (PAA) or a strong polyacid-poly(2-acrylamido-2-methylpropanesulfonic acid) (PAMPS). The quaternization of the amino groups of chitosan enabled the formation of water-insoluble PECs in a broad pH range—from 3 to 12 and from 1 to 12 for TMCh/PAA and TMCh/PAMPS, respectively. Whereas the stoichiometry of the TMCh/PAA complex was pH dependant, the stoichiometry of the TMCh/PAMPS complex did not depend on pH. The stoichiometry and the yield of the complexes were influenced by the molar mass of TMCh. PEC nanoparticles were produced by mixing dilute solutions of the oppositely charged polyelectrolytes as revealed by dynamic light scattering analyses. The size of the particles was in the range of 135–924 nm and depended on the polyelectrolyte molar mass, the initial polyelectrolyte concentration, and the molar fraction of the TMCh units. Microbiological screening against Staphylococcus aureus and Escherichia coli revealed that PECs between TMCh and PAA or PAMPS have a good antibacterial effect, which is more slowly pronounced than that of the starting TMCh of different molar mass.

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Acknowledgments

Financial support from the National Science Fund (Grant DCVP 02/2–UNION) is kindly acknowledged. KK acknowledges the OP-HRD Grant BG051PO001-3.3.06-006 of the European Social Fund.

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Authors and Affiliations

  1. Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, 1113, Sofia, Bulgaria

    Kalin Kalinov, Milena Ignatova, Nevena Manolova & Iliya Rashkov

  2. Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Bl. 26, 1113, Sofia, Bulgaria

    Nadya Markova

  3. Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav Street, 1000, Sofia, Bulgaria

    Denitsa Momekova

Authors
  1. Kalin Kalinov
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  2. Milena Ignatova
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  3. Nevena Manolova
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Correspondence to Nevena Manolova.

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Kalinov, K., Ignatova, M., Manolova, N. et al. N,N,N-trimethylchitosan iodide complexes with a weak or a strong polyacid and nanoparticles thereof. Colloid Polym Sci 292, 2899–2912 (2014). https://doi.org/10.1007/s00396-014-3325-6

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  • DOI: https://doi.org/10.1007/s00396-014-3325-6

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