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Moderate physical training attenuates perinatal low-protein-induced spleen lymphocyte apoptosis in endotoxemic adult offspring rats

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Abstract

Purpose

To evaluate the effects of a moderate physical training (T) on the blood and splenic lymphocytes subsets and the rate of apoptosis in adult offspring submitted to perinatal low-protein (LP) diet.

Methods

Male Wistar rats were divided according to their mother’s diet: control (C, 17 % casein) and undernourished (LP, 8 % casein). At the 60th day, pups were submitted to moderate physical training (8 weeks, 5 days week−1, 60 min day−1, at 70 % of VO2max). After T period, pups received an injection of lipopolysaccharide (LPS). B, NK, and TCD3+ lymphocytes subsets were analyzed by flow cytometry. Spleen lymphocytes apoptosis was evaluated by DNA fragmentation, phosphatidylserine externalization (PSE), and mitochondrial transmembrane depolarization (MTD) using a flow cytometer. Plasma TNF-α concentrations were analyzed by ELISA.

Results

LP + LPS pups showed a higher percentage of blood B, CD4+, and NK and a reduction in TCD3+, CD8+ than C pups. The percentage of NK and CD3+ was restored in LP + T + LPS pups. In the spleen, T normalized the percentage of NK in LP + LPS pups. LP + LPS pups showed a higher percentage of cells with PSE and MTD than C + LPS pups that was attenuated by T. The concentration of TNF-α was higher in LP + LPS than C + LPS, but it was attenuated in LP + T + LPS pups.

Conclusion

Moderate physical training was able to revert the effects of perinatal LP diet on circulation lymphocytes subsets and attenuated splenic lymphocytes apoptosis and plasma TNF-α concentrations.

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Acknowledgments

This study was supported by National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Level or Education Personnel (CAPES), and State of Pernambuco Science and Technology Support Foundation (FACEPE).

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Nutrition, Federal University of Pernambuco, Recife, Brazil

    Sueli Moreno Senna, Marília Kalinne Torres, Daíllo Augusto Pereira Lopes, Maria Claudia Alheiros-Lira, Diógenis Barbosa de Moura & Carol Góis Leandro

  2. Department of Nursing, Academic Center of Vitoria de Santo Antao, Federal University of Pernambuco, Vitória de Santo Antão, Brazil

    Sueli Moreno Senna & Francisco Carlos Amanajás de Aguiar Jr.

  3. Departament of Immunology, Institute of Research Aggeu Magalhães, FIOCRUZ/PE, Recife, Brazil

    Valéria Rêgo Alves Pereira

  4. Department of Physical Education and Sport Science, Academic Center of Vitoria de Santo Antao, Federal University of Pernambuco, Rua Alto do Reservatório, s/n, Bela Vista, Vitória de Santo Antão, PE, CEP 55608-680, Brazil

    José Candido Ferraz & Carol Góis Leandro

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  1. Sueli Moreno Senna
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Correspondence to Carol Góis Leandro.

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Senna, S.M., Torres, M.K., Lopes, D.A.P. et al. Moderate physical training attenuates perinatal low-protein-induced spleen lymphocyte apoptosis in endotoxemic adult offspring rats. Eur J Nutr 55, 1113–1122 (2016). https://doi.org/10.1007/s00394-015-0925-y

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  • DOI: https://doi.org/10.1007/s00394-015-0925-y

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