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Lactobacillus plantarum CECT 7315/7316 intake modulates the acute and chronic innate inflammatory response

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Abstract

Purpose

Probiotics may confer health benefits for the host. Although Lactobacillus has demonstrated to stimulate the immune response, only a few strains have demonstrated immunomodulatory properties. The newly identified Lactobacillus plantarum strains CECT7315 and CECT7316 (LP3457) seem to boost the immune system in individuals that immune decline. We aimed to investigate whether LP3457 protects against inflammation and the mechanism behind.

Methods

LP3457 potential anti-inflammatory effects were assessed in an acute model LPS-induced inflammation in healthy rats and in a chronic model of low-grade inflammation in Zucker diabetic fatty (ZDF) rats. Wistar rats received LP3457 or placebo control for 20 days. Lipopolysaccharide (LPS; 1 mg/kg) was injected intraperitoneally at day 14, and animals were sacrificed 6 days after. Blood was collected at baseline (day 0) and consecutively at day 7, 14, 17, and 20 for haematological evaluation and assessment of anti-inflammatory/pro-inflammatory systemic markers. Myeloperoxidase activity was investigated in the ileum. ZDF rats received LP3457 or placebo control during 8 weeks, and changes in inflammasome-related transcripts were assessed in the ileum.

Results

LPS induced a comparable and significant leucocytosis 3 days post-injection (day 17) in both LP3457-treated and LP3457-untreated rats. However, the probiotic supplementation attenuated IL-1β, IL-6, and CRP release and increased anti-inflammatory IL-10 levels 6 days post-LPS induction (p < 0.05 vs. placebo). LP3457-supplemented animals also displayed lower intestinal myeloperoxidase activity (p < 0.05 vs. placebo). Chronic administration of LP3457 to ZDF rats resulted in a significant downregulation of the inflammasome signalling pathway (p < 0.05 vs. placebo).

Conclusions

Intake of LP3457 attenuates both acute endotoxemia-induced and chronic metabolically induced inflammatory reactions and the inflammasome signalling pathway. The stabilization and regulation of the gut microbiota is an important target for reducing the impact of organ-related inflammatory reactions.

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Acknowledgments

The present study was supported by the company CARINSA Group through HENUFOOD Project (CEN-20101016) which is part of the CENIT program from Spanish Minister of Economy and Competitiveness. This work was also supported by the Health National Program SAF2013-42962-R (to L.B.) and SAF 2012-40208 (to G.V.) from the Spanish Ministry of Science; Institute Carlos III (to L.B.); and CEN-20101016 (HENUFOOD) CDTI-Spanish Ministry of Competitivity and Economy (MINECO) (to L.B.). We thank Fundación Jesus Serra, Barcelona, for their continuous support. G.V. is a recipient of a contract from the Innovation and Science Spanish Ministry (RyC-2009-5495, MICINN, Spain). The authors also thank Josep Moreno for their technical support.

Conflict of interest

The authors declare that they have no conflict of interest. All authors have read and approved the final manuscript.

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Authors and Affiliations

  1. Cardiovascular Research Center, CSIC-ICCC, Hospital de la Santa Creu i Sant Pau, IIB-Sant Pau, C/Sant Antoni Mª Claret 167, 08025, Barcelona, Spain

    Gemma Vilahur, Sergi López-Bernal, Sandra Camino, Guiomar Mendieta, Teresa Padró & Lina Badimon

  2. Cardiovascular Research Chair, UAB, Barcelona, Spain

    Lina Badimon

Authors
  1. Gemma Vilahur
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  2. Sergi López-Bernal
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  3. Sandra Camino
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  4. Guiomar Mendieta
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  5. Teresa Padró
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  6. Lina Badimon
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Correspondence to Lina Badimon.

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Gemma Vilahur and Sergi López-Bernal have contributed equally to the work.

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Vilahur, G., López-Bernal, S., Camino, S. et al. Lactobacillus plantarum CECT 7315/7316 intake modulates the acute and chronic innate inflammatory response. Eur J Nutr 54, 1161–1171 (2015). https://doi.org/10.1007/s00394-014-0794-9

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