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Association between the ACE gene I/D polymorphism and osteoporosis in a Turkish population

Verbindung zwischen dem I/D-Polymorphismus des ACE-Gens und Osteoporose in einer türkischen Population

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Abstract

Purpose

The purpose of this study was to assess the relationship between osteoporosis and the angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism in a Turkish population.

Methods

The study group consisted of 238 unrelated women with osteoporosis and 124 unrelated healthy female controls. All participants, patients and healthy controls, were of Turkish origin from the central region of Turkey. Genomic DNA was isolated from whole venous blood samples using a commercial DNA isolation kit. The ACE gene I/D polymorphism was analysed by polymerase chain reaction and gel electrophoresis.

Results

Frequencies of the DD, ID and II genotypes in the patients were 44.5, 41.2 and 14.3 %, and in the controls they were 25.0, 51.6 and 23.4 %, respectively. A significant difference was observed between patients and controls according to genotype frequency (p = 0.001). D and I allele frequencies of the I/D polymorphism were 65.1 and 34.9 % in the patient group and 50.8 and 49.2 % in the control group, respectively (p < 0.001).

Conclusion

The ACE gene I/D polymorphism could be a genetic factor associated with osteoporosis.

Zusammenfassung

Ziel

Ziel dieser Studie war es, die Verbindung zwischen Osteoporose und dem Insertion/Deletion(I/D)-Polymorphismus des Angiotensin-konvertierenden-Enzym(ACE)-Gens in einer türkischen Population zu untersuchen.

Methoden

Die Studiengruppe bestand aus 238 nichtverwandten Frauen mit Osteoporose und einer Kontrollgruppe mit 124 nichtverwandten gesunden Frauen. Alle Teilnehmenden, die Patienten und die gesunde Kontrollgruppe, stammten aus der Zentraltürkei. Mittels eines handelsüblichen DNA-Isolationskits wurde aus venösen Blutproben genomische DNA entnommen. Der ACE-I/D-Polymorphismus wurde mittels Polymerase-Kettenreaktion und Gelelektrophorese analysiert.

Ergebnisse

Die Häufigkeiten der Genotypen DD, ID und II in den Patienten waren 44,5, 41,2 und 14,3 %, in der Kontrollgruppe betrugen sie 25,0, 51,6 und 23,4 %. Es wurde ein signifikanter Unterschied zwischen den Patienten und der Kontrollgruppe bezüglich der Genotyp-Häufigkeit (p = 0,001) beobachtet. Die Häufigkeiten der Allelen D und I der I/D-Polymorphismen betrugen 65,1 und 34,9 % in der Patientengruppe bzw. 50,8 und 49,2 % in der Kontrollgruppe (p < 0,001).

Conclusion

Beim I/D-Polymorphismus des ACE-Gens könnte es sich um einen genetischen Faktor handeln, der mit Osteoporose assoziiert ist.

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Compliance with ethical guidelines

Conflict of interest. B. Cakmak, A. Inanir, N. Karakus, O. Ates and S. Yigit state that there are no conflicts of interest.

All studies on humans described in the present manuscript were carried out with the approval of the responsible ethics committee and in accordance with national law and the Helsinki Declaration of 1975 (in its current, revised form). Informed consent was obtained from all patients included in studies.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Gaziosmanpasa University School of Medicine, Sevki Erek Yerleskesi, 60100, Tokat, Turkey

    B. Cakmak MD.

  2. Department of Physical Therapy and Rehabilitation, Gaziosmanpasa University School of Medicine, Tokat, Turkey

    A. Inanir MD.

  3. Department of Medical Biology, Gaziosmanpasa University School of Medicine, Tokat, Turkey

    N. Karakus PhD., O. Ates PhD. & S. Yigit PhD.

Authors
  1. B. Cakmak MD.
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  2. A. Inanir MD.
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  3. N. Karakus PhD.
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  4. O. Ates PhD.
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  5. S. Yigit PhD.
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Correspondence to B. Cakmak MD..

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Cakmak, B., Inanir, A., Karakus, N. et al. Association between the ACE gene I/D polymorphism and osteoporosis in a Turkish population. Z Rheumatol 74, 346–350 (2015). https://doi.org/10.1007/s00393-015-1582-5

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  • DOI: https://doi.org/10.1007/s00393-015-1582-5

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