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The osteogenic potential of ligament fibroblasts is greater in ankylosing spondylitis patients than in patients with osteoarthritis

Größeres osteogenes Potenzial von ligamentären Fibroblasten bei Patienten mit Spondylitis ankylosans als bei Patienten mit Arthrose

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Abstract

Background

The goal of the present study was to investigate the osteogenic potential of ligament fibroblasts from patients with ankylosing spondylitis (AS).

Materials and methods

Ligament fibroblasts were isolated from tissues harvested from AS patients and patients with aseptic necrosis of the femoral head (ANFH) who had undergone total hip arthroplasty (THA). Cells were cultured in osteogenic induction medium (OIM) with or without bone morphogenetic protein 2 (BMP-2) for 30 days. During this time, the levels of alkaline phosphatase (ALP) and osteocalcin (OC) were determined as markers of osteogenesis.

Results

Ligament fibroblasts isolated from patients with AS exhibited significantly greater potential for differentiation than those isolated from patients with osteoarthritis (ANFH).

Conclusion

These findings illuminate osteogenesis as a new pathway for studying the mechanism underlying ankylosis in AS patients. Factors which cause localized stromal remodeling at the enthesis significantly influence formation of new bone and further research is required to determine the mechanisms responsible for the osteogenic potential of enthesis cells and ligament fibroblasts in AS patients.

Zusammenfassung

Hintergrund

Ziel der vorliegenden Studie war es, das osteogene Potenzial ligamentärer Fibroblasten von Patienten mit Spondylitis ankylosans (AS) zu untersuchen,

Material und Methoden

Ligamentäre Fibroblasten wurden aus Gewebe von AS-Patienten und Patienten mit aseptischer Femurkopfnekrose mit Zustand nach Totalendoprothese (TEP) der Hüfte isoliert. Die Zellen wurden 30 Tage in osteogenem Induktionsmedium (OIM) mit oder ohne BMP-2 („bone morphogenetic protein 2“) kultiviert. Währenddessen wurden alkalische Phosphatase (ALP) und Osteocalcin (OC) als Osteogenesemarker bestimmt.

Ergebnisse

Von AS-Patienten isolierte ligamentäre Fibroblasten wiesen ein signifikant größeres Differenzierungspotenzial auf als Fibroblasten von Arthrosepatienten.

Schlussfolgerung

Die vorliegenden Ergebnisse zeigen die Osteogenese als neuen Signalweg zur Untersuchung der Pathophysiologie der Ankylose bei AS-Patienten. Faktoren, die ein lokalisiertes stromales Remodeling am Sehnenansatz verursachen, wirken sich signifikant auf die Bildung neuen Knochens aus. Um die Vorgänge zu untersuchen, die das osteogene Potenzial der Zellen des Sehnenansatzes und der ligamentären Fibroblasten bei AS-Patienten beeinflussen, sind weitere Studien erforderlich.

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Acknowledgments

This work was supported by the Shanghai Key Basic Research Project (No. 10JC1417900).

Compliance with ethical guidelines

Conflict of interest. M. Yang, H. Yuan, M. Miao and W. Xu state that there are no conflicts of interest. All studies on humans described in the present manuscript were carried out with the approval of the responsible ethics committee and in accordance with national law and the Helsinki Declaration of 1975 (in its current, revised form). Informed consent was obtained from all patients included in studies.

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Authors and Affiliations

  1. Department of Orthopedics, Changhai Hospital, the Second Military Medical University, 200433, Shanghai, P. R. China

    M. Yang & W. Xu

  2. Department of Orthopedics, Zhongshan Hospital, Shanghai Medical College of Fudan University, Shanghai, P. R. China

    H. Yuan

  3. Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, P. R. China

    M. Miao

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  1. M. Yang
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  2. H. Yuan
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  3. M. Miao
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  4. W. Xu
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Corresponding author

Correspondence to W. Xu.

Additional information

Yang Muyi and Yuan Hengfeng contributed equally to this work.

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Yang, M., Yuan, H., Miao, M. et al. The osteogenic potential of ligament fibroblasts is greater in ankylosing spondylitis patients than in patients with osteoarthritis. Z Rheumatol 74, 340–345 (2015). https://doi.org/10.1007/s00393-014-1394-z

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  • DOI: https://doi.org/10.1007/s00393-014-1394-z

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