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Prognostic significance of AEG-1 expression in colorectal carcinoma

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Background

Astrocyte elevated gene-1 (AEG-1), as an HIV-1 or TNF-α-inducible transcript, is associated with various aspects of tumor malignancy. However, relatively little knowledge is available related to the role of AEG-1 in colorectal carcinoma.

Methods

By immunohistochemical and western blot analysis, we investigated AEG-1 expression in normal mucosa, adenomas, and carcinomas of colorectum. By statistical analysis, we determined its relationship with clinicopathological parameters and overall survival in colorectal carcinoma.

Results

We found that AEG-1 expression levels were gradually elevated in normal tissues, low-grade adenoma, high-grade adenoma, and colorectal carcinoma, respectively. Though AEG-1 staining mainly emerged in the cytoplasm, we observed that nuclear staining of AEG-1 tends to become more common in lesions from patients with more advanced disease stages. Furthermore, there was a similar trend for Ki67 expression (as a proliferative index) from normal mucous to adenoma and carcinoma. Statistical analysis revealed that AEG-1 expression was markedly correlated with the UICC stage (P < 0.001), T classification (P = 0.002), N classification (P = 0.015), M classification (P = 0.010), Ki67 expression (P = 0.012), and histological differentiation (P = 0.037) in the colorectal cancer patients. Besides, those patients with high AEG-1 levels had shorter survival time (P < 0.001).

Conclusions

High AEG-1 expression correlates with poor overall survival in the colorectal carcinoma patients. In addition, AEG-1 expression in colorectal carcinoma may be associated with tumor progression, indicating that AEG-1 may be a potential preventive and chemotherapeutic target in the patients.

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Abbreviations

AEG-1:

Astrocyte-elevated gene-1

HIV-1:

Human immunodeficiency virus type 1

TNF-α:

Tumor necrosis factor-α

PI3K:

Phosphoinositide 3-kinase

NF–κB:

Nuclear factor–kappa B

AP-1:

Activator protein 1

ERK:

Extracellular signal-regulated protein kinase

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Acknowledgements

We would like to thank Aiwen Hou for her support and help in the preparation of this manuscript. We thank Jing Bai and Qiuju Xu for their technical assistance. This research is funded by Natural Science Foundation of Heilongjiang Province (No. D2009-21) and Innovation Fund for Excellent Graduates of Harbin Medical University (No. HCXB2010018).

Conflict of interest

We have no conflict of interest existing.

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Authors and Affiliations

  1. Department of Pathology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, 150040, China

    Hongtao Song, Cong Li & Jingshu Geng

  2. Department of Medical Genetics, Harbin Medical University, Harbin, 150086, China

    Jingshu Geng

  3. Department of Surgery, People’s Hospital, Peking University, Beijing, 100044, China

    Rui Li

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  1. Hongtao Song
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  2. Cong Li
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  3. Rui Li
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  4. Jingshu Geng
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Corresponding author

Correspondence to Jingshu Geng.

Additional information

Hongtao Song and Cong Li contributed equally to this manuscript.

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Song, H., Li, C., Li, R. et al. Prognostic significance of AEG-1 expression in colorectal carcinoma. Int J Colorectal Dis 25, 1201–1209 (2010). https://doi.org/10.1007/s00384-010-1009-3

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  • DOI: https://doi.org/10.1007/s00384-010-1009-3

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