Denitrification, N₂O and CO₂ fluxes in rice-wheat cropping system as affected by crop residues, fertilizer N and legume green manure

Abstract.

Use of renewable N and C sources such as green manure (GM) and crop residues in rice-wheat cropping systems of South Asia may lead to higher crop productivity and C sequestration. However, information on measurements of gaseous N losses (N₂O+N₂) via denitrification and environmental problems such as N₂O and CO₂ production in rice-wheat cropping systems is not available. An acetylene inhibition-intact soil core technique was employed for direct measurement of denitrification losses, N₂O and CO₂ production, in an irrigated field planted to rice (Oryza sativa L.) and wheat (Triticum aestivum L.) in an annual rotation. The soil was a coarse-textured Tolewal sandy loam soil (Typic Ustochrept) and the site a semi-arid subtropical Punjab region of India. Wheat residue (WR, C:N=94) was incorporated at 6 t ha^–1 and sesbania (Sesbania aculeata L.) was grown as GM crop for 60 days during the pre-rice fallow period. Fresh biomass of GM (C:N.=18) at 20 or 40 t ha^–1 was incorporated into the soil 2 days before transplanting rice. Results of this study reveal that (1) denitrification is a significant N loss process under wetland rice amounting to 33% of the prescribed dose of 120 kg N ha^–1 applied as fertilizer urea-N (FN); (2) integrated management of 6 t WR ha^–1 and 20 t GM ha^–1 supplying 88 kg N ha^–1 and 32 kg FN ha^–1 significantly reduced cumulative gaseous N losses to 51.6 kg N ha^–1 as compared with 58.2 kg N ha^–1 for 120 kg FN ha^–1 alone; (3) application of excessive N and C through applying 40 t GM ha^–1 (176 kg N ha^–1) resulted in the highest gaseous losses of 70 kg N ha^–1; (4) the gaseous N losses under wheat were 0.6% to 2% of the applied 120 kg FN ha^–1 and were eight- to tenfold lower (5–8 kg N ha^–1) than those preceding rice; (5) an interplay between the availability of NO₃ ^– and organic C largely controlled denitrification and N₂O flux during summer-grown flooded rice whereas temperature and soil aeration status were the primary regulators of the nitrification-denitrification processes and gaseous N losses during winter-grown upland wheat; (6) the irrigated rice-wheat system is a significant source of N₂O as it emits around 15 kg N₂O-N ha^–1 year^–1; (7) incorporation of WR in rice and rice residue (C:N=63) in wheat increased soil respiration, and increased CO₂ production in WR- and GM-amended soils under anaerobic wetland rice coincided with enhanced rates of denitrification; and (8) with adequate soil moisture, most of the decomposable C fraction of added residues was mineralized within one crop-growing season and application of FN and GM further accelerated this process.