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A novel phosphorus biofertilization strategy using cattle manure treated with phytase–nanoclay complexes

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Abstract

The aim of this work was to evaluate the treatment of cattle manure with phytases stabilized in allophanic nanoclays as a potential novel phosphorus (P) biofertilization technology for crops grown in volcanic soils (Andisol). Furthermore, because the optimal pH for commercial phytase catalysis does not match the natural pH of manure, a complementary experiment was set up to evaluate the effect of manure inoculation with an alkaline phytase-producing bacterium. Finally, phytase-treated soil, manure, and soil–manure mixtures were evaluated for their P-supplying capacity to wheat plants grown under greenhouse conditions. Treating cattle manure with phytases stabilized in nanoclays resulted in a significant (P ≤ 0.05) increase of inorganic P in soil extracts (NaOH-EDTA and Olsen). The use of phytase-treated cattle manure increased dry weights by 10 % and the P concentration by 39 % in wheat plants grown under greenhouse conditions, which is equivalent to a P fertilizer rate of about 150 kg of P per hectare. The inoculation of cattle manure with β-propeller phytase-producing bacteria led to an ∼10 % increase in inorganic P in the manure extracts. However, applying inoculated manure to soil did not significantly increase wheat yield or P acquisition responses. Our results suggest that the novel approach of incubating cattle manure with phytases stabilized in nanoclay enhances the organic P cycling and P nutrition of plants grown in P-deficient soils.

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Acknowledgments

This work was financed by FONDECYT research projects no 1100625 and no 1120505. D. Menezes-Blackburn acknowledges to the following financial supports: UFRO and CONICYT Doctoral Scholarships, and Georg Forster Research Postdoctoral Fellowship (Humboldt Foundation). MA Jorquera also thanks to the support from International Cooperation Research CONICYT-BMBF code 2009-183.

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Authors and Affiliations

  1. Chile–Italy International Doctorate in Environmental Resources Science, Universidad de La Frontera, Ave.nida Francisco Salazar, 01145, Temuco, Chile

    Daniel Menezes-Blackburn

  2. Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Avenida Francisco Salazar, 01145, Temuco, Chile

    Milko A. Jorquera & María de la Luz Mora

  3. Dipartimento di Scienze del Suolo, della Pianta e dell’Ambiente, e delle Produzioni Animali, Università di Napoli, Federico II, Portici, Italy

    Liliana Gianfreda

  4. Department of Food Technology and Bioprocess Engineering, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany

    Daniel Menezes-Blackburn & Ralf Greiner

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  1. Daniel Menezes-Blackburn
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  2. Milko A. Jorquera
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  3. Liliana Gianfreda
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  4. Ralf Greiner
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  5. María de la Luz Mora
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Correspondence to María de la Luz Mora.

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Menezes-Blackburn, D., Jorquera, M.A., Gianfreda, L. et al. A novel phosphorus biofertilization strategy using cattle manure treated with phytase–nanoclay complexes. Biol Fertil Soils 50, 583–592 (2014). https://doi.org/10.1007/s00374-013-0872-9

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  • DOI: https://doi.org/10.1007/s00374-013-0872-9

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