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Increased Polyamines Conjugated to Tonoplast Vesicles Correlate with Maintenance of the H+-ATPase and H+-PPase Activities and Enhanced Osmotic Stress Tolerance in Wheat

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Abstract

The effects of osmotic stress on H+-ATPase and H+-PPase activities and the levels of covalently conjugated polyamines (CC-PAs) and noncovalently conjugated polyamines (NCC-PAs) were investigated using tonoplast vesicles isolated from the roots of wheat (Triticum aestivum L.) seedlings differing in drought-tolerance. The results showed that after polyethylene glycol (PEG) 6,000 (−0.55MPa) treatment for 7 days, seedling leaf relative water content (LRWC), relative dry weight increase rate (RDWIR) and root H+-ATPase and H+-PPase activities from the drought-sensitive cultivar Yangmai No. 9 decreased more markedly than those from the drought-tolerant cultivar Yumai No. 18. At the same time, the increase of the NCC-spermidine (NCC-Spd) and CC-putrescine (CC-Put) levels in root tonoplast vesicles from Yumai No. 18 was more obvious than that from Yangmai No. 9. Exogenous Spd treatment alleviated osmotic stress injury to Yangmai No. 9 seedlings, coupled with marked increases of tonoplast NCC-Spd levels and H+-ATPase and H+-PPase activities. Treatments with methylglyoxyl bis (guanyl hydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC), and phenanthrolin, an inhibitor of transglutaminase (TGase), significantly inhibited the osmotically induced increases of NCC-Spd and CC-Put levels, respectively, in root tonoplast vesicles from Yumai No. 18 seedlings. Both MGBG and phenanthrolin treatments markedly promoted osmotically induced decreases of tonoplast H+-ATPase and H+-PPase activities and osmotic stress tolerance of seedlings of this cultivar. These results suggest that the NCC-Spd and CC-Put present in tonoplast vesicles isolated from wheat seedling roots might enhance the adaptation of seedlings to osmotic stress via maintenance of tonoplast H+-ATPase and H+-PPase activities.

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Acknowledgments

This research was supported by the Research Foundation especially for Doctoral Programs at the Chinese University (20020307004), the National High Science and Technology Program (863) (2002AA2Z4061) and the National Natural Science Foundation of China (30370850). We also thank professor Weixing Cao and Guangyu Yang for their kindly gifts of wheat seeds.

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Authors and Affiliations

  1. College of Life Science, Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China

    H. Liu, Y. Liu, B. Yu, Z. Liu & W. Zhang

  2. Department of Biology, Zhoukou Normal College, Henan Province, 466000, China

    H. Liu

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  1. H. Liu
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Correspondence to Y. Liu.

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Liu, H., Liu, Y., Yu, B. et al. Increased Polyamines Conjugated to Tonoplast Vesicles Correlate with Maintenance of the H+-ATPase and H+-PPase Activities and Enhanced Osmotic Stress Tolerance in Wheat. J Plant Growth Regul 23, 156–165 (2004). https://doi.org/10.1007/s00344-004-0072-9

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