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Cloning of the cDNA encoding adenosine 5′-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus

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Abstract

AMP deaminase catalyzes the conversion of AMP into IMP and ammonia. In the present study, a full-length cDNA of AMPD1 from skeletal muscle of Japanese flounder Paralichthys olivaceus was cloned and characterized. The 2 526 bp cDNA contains a 5′-UTR of 78 bp, a 3′-UTR of 237 bp and an open reading frame (ORF) of 2 211 bp, which encodes a protein of 736 amino acids. The predicted protein contains a highly conserved AMP deaminase motif (SLSTDDP) and an ATP-binding site sequence (EPLMEEYAIAAQVFK). Phylogenetic analysis showed that the AMPD1 and AMPD3 genes originate from the same branch, but are evolutionarily distant from the AMPD2 gene. RT-PCR showed that the flounder AMPD1 gene was expressed only in skeletal muscle. QRT-PCR analysis revealed a statistically significant 2.54 fold higher level of AMPD1 mRNA in adult muscle (750±40 g) compared with juvenile muscle (7.5±2 g) (P<0.05). HPLC analysis showed that the IMP content in adult muscle (3.35±0.21 mg/g) was also statistically significantly higher than in juvenile muscle (1.08±0.04 mg/g) (P<0.05). There is a direct relationship between the AMPD1 gene expression level and IMP content in the skeletal muscle of juvenile and adult flounders. These results may provide useful information for quality improvement and molecular breeding of aquatic animals.

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Authors and Affiliations

  1. R&D Center of Marine Biotechnology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Keyong Jiang  (蒋克勇), Shujuan Sun  (孙姝娟), Mei Liu  (刘梅), Baojie Wang  (王宝杰), Xiaolin Meng  (孟晓林) & Lei Wang  (王雷)

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Keyong Jiang  (蒋克勇) & Xiaolin Meng  (孟晓林)

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  1. Keyong Jiang  (蒋克勇)
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  2. Shujuan Sun  (孙姝娟)
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  3. Mei Liu  (刘梅)
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  4. Baojie Wang  (王宝杰)
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  5. Xiaolin Meng  (孟晓林)
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  6. Lei Wang  (王雷)
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Correspondence to Lei Wang  (王雷).

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Supported by the National Natural Science Foundation of China (No. 41206144) and the National High Technology Research and Development Program of China (863 Program) (No. 2008AA100805)

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Jiang, K., Sun, S., Liu, M. et al. Cloning of the cDNA encoding adenosine 5′-monophosphate deaminase 1 and its mRNA expression in Japanese flounder Paralichthys olivaceus . Chin. J. Ocean. Limnol. 31, 118–127 (2013). https://doi.org/10.1007/s00343-013-2055-9

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  • DOI: https://doi.org/10.1007/s00343-013-2055-9

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