Abstract
DNA double-strand breaks (DSBs) are produced intentionally by RNA-guided nucleases to achieve genome editing through DSB repair. These breaks are repaired by one of two main repair pathways, classic non-homologous end joining (c-NHEJ) and homology-directed repair (HDR), the latter being restricted to the S/G2 phases of the cell cycle and notably less frequent. Precise genome editing applications rely on HDR, with the abundant c-NHEJ formed mutations presenting a barrier to achieving high rates of precise sequence modifications. Here, we give an overview of HDR- and c-NHEJ-mediated DSB repair in gene editing and summarize the current efforts to promote HDR over c-NHEJ.
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Funding was provided by BMBF (Grant No: VIP 03V0261).
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Eric Danner and Sanum Bashir have contributed equally to this work.
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Danner, E., Bashir, S., Yumlu, S. et al. Control of gene editing by manipulation of DNA repair mechanisms. Mamm Genome 28, 262–274 (2017). https://doi.org/10.1007/s00335-017-9688-5
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DOI: https://doi.org/10.1007/s00335-017-9688-5