Abstract
Quantitative sampling of zooplankton communities from ice-covered waters presents many technical and logistic difficulties. Currently available techniques enable only vertical tows through relatively small ice holes, and the filtered volumes are generally low. For these reasons, we developed and tested Micro-Net Environmental Sampling System (MicroNESS), an innovative small-sized opening–closing multinet device carried by a ROV for horizontal and oblique sampling of the under-ice zooplankton community. MicroNESS design ensures a filtration efficiency close to 90 % due to the relevant filtration open area ratio (~4) and exhibited only limited effects of clogging and avoidance for optimal speed of 0.35 ms−1 and towing duration not longer than 14 min with mean filtered volumes in the order of 11 m3. MicroNESS was successfully tested in Terra Nova Bay (Antarctica). A total of 96 tows were carried out to sample the zooplankton in the waters below the 2.6-m-thick pack ice, far from the ice hole, at selected depths (3.5, 4.5, 5.0, 6.0, 7.0, 10.0 and 20.0 m). Overall, more than 70,000 specimens were identified, mostly copepod taxa, which showed a size range from the small Stephos longipes nauplius stage I (0.11 mm) to the large adult females of Calanoides acutus (4.8 mm). The comparison among the ice-associated copepod communities collected by MicroNESS and those of three traditional sampling techniques showed that MicroNESS can provide useful insights about changes in composition, biomass and particle size structure of this key plankton component, especially on a short timescale during ice melting.
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References
Archer SD, Leakey RJG, Burkill PH, Sleigh MA (1996) Microbial dynamics in coastal waters of East Antarctica herbivory by heterotrophic dinoflagellates. Mar Ecol Prog Ser 139:239–255
Arndt CE, Swadling KM (2006) Crustacea in Arctic and Antarctic sea ice: distribution, diet and life history strategies. Adv Mar Biol 51:197–315
Bayly IAE (1986) Ecology of the zooplankton of a meromictic antarctic lagoon with special reference to Drepanopus bispinosus (Copepoda: calanoida). Hydrobiologia 140:199–231
Caccia M, Bono R, Bruzzone G, Verruggio G (1999) Variable configuration UUVs for marine science applications. Robot Autom Mag IEEE 6(2):22–32
Caccia M, Bono R, Bruzzone G, Verruggio G (2000) Unmanned underwater vehicles for scientific applications and robotic research: the ROMEO project. Mar Tech Soc J 34(2):3–17
Clutter RI, Anraku M (1968) Avoidance of samplers. In: Tranter DJ (ed) Zooplankton sampling. Monographs on oceanographic methodology. UNESCO, Paris, pp 57–76
Delille D, Rosiers C (1996) Seasonal changes of Antarctic marine bacterioplankton and sea ice bacterial assemblages. Polar Biol 16:27–34
Fleminger A, Clutter RI (1965) Avoidance of towed nets by zooplankton. Limnol Oceanogr 10:96–104
Gradinger R (1999) Integrated abundance and biomass of sympagic meiofauna in Arctic and Antarctic pack ice. Polar Biol 22:169–177
Guglielmo L, Carrada GC, Catalano G, Dell’Anno A, Fabiano M, Lazzara L, Mangoni O, Pusceddu A, Saggiomo V (2000) Structural and functional properties of sympagic communities in the annual sea ice at Terra Nova Bay (Ross Sea, Antarctica). Polar Biol 23:137–146
Guglielmo L, Zagami G, Saggiomo V, Catalano G, Granata A (2007) Copepods in spring annual sea ice at Terra Nova Bay (Ross Sea, Antarctica). Polar Biol 30:747–758
Heron AC (1982) A vertical free—fall plankton net with no mouth obstructions. Limnol Oceanogr 27:380–383
Hoerner SF (1965) Fluid-dynamic drag: practical information on aerodynamic drag and hydrodynamic resistance. Hoerner, Midland Park, pp 1–452
Kirkwood JM, Burton HR (1987) Three new zooplankton nets designed for under-ice sampling; with preliminary results of collections made from Ellis Fjord, Antarctica during 1985. Proc NIPR Symp Polar Biol 1:112–122
Lambeck K, Esat TM, Potter E-K (2002) Links between climate and sea levels for the past three million years. Nature 419:199–206
Legendre P, Legendre L (1998) Numerical ecology, 2nd English edn. Elsevier, Amsterdam, pp 1–853
Loots C, Swadling KM, Koubbi P (2009) Annual cycle of distribution of three ice-associated copepods along the coast near Dumont d’Urville, Terre Adélie (Antarctica). J Mar Syst 78:599–605
Macaulay MC, Daly KL (1987) A collapsible opening-closing net for zooplankton sampling through ice. J Plankton Res 9:1069–1073
McGowan JA, Raundorf VJI (1966) The relationship between size of net used and estimates of zooplankton diversity. Limnol Oceanogr 11:456–469
Rakusa-Suszczewski S (1972) The biology of Paramoera walkeri Stebbing (amphipoda) and the Antarctic sub-fast ice community. Pol Arch Hydrobiol 19:11–36
Rand K, Logerwell E (2009) Through-ice sampling workshop. AFSC Processed Rep. 2009–02. Alaska Fish Sci Cent, NOAA, Natl Mar Fish Serv, 7600 Sand Point Way NE, Seattle WA 98115
Sameoto DD, Jaroszynski LO, Fraser WB (1980) BIONESS, a new design in multiple net zooplankton samplers. Can J Fish Aquat Sci 37:722–724
Saville A (1958) Mesh selection in plankton nets. J Cons Perm Int Explor Mer 23:192–201
Schnack-Schiel SB, Thomas DN, Haas C, Dieckmann GS, Alheit R (2001) The occurrence of the copepods Stephos longipes (Calanoida) and Drescheriella glacialis (Harpacticoida) in summer sea ice in the Weddell Sea, Antarctica. Antarct Sci 13:150–157
Schnack-Schiel SB, Dieckmann G, Kattner G, Thomas DN (2004) Copepods in summer platelet ice in the eastern Weddell Sea. Polar Biol 27:502–506
Smith PE, Counts RC, Clutter RI (1968) Changes in filtering efficiencies of plankton nets due to clogging under tow. J Cons Perm Int Explor Mer 32:232–248
Spindler M (1994) Notes on the biology of sea ice in the Arctic and Antarctic. Polar Biol 14:319–324
Spindler M, Dieckmann GS, Lange MA (1990) Seasonal and geographic variations in sea ice community structure of the Weddell Sea, Antarctica. In: Kerry KR, Hempel G (eds) Antarctic ecosystems, ecological change and conservation. Springer, Berlin, pp 129–135
Swadling KM, McKinnon AD, De’ath G, Gibson JAE (2004) Life cycle plasticity and differential growth and development in marine and lacustrine populations of an Antarctic copepod. Limnol Oceanogr 49:644–655
Tranter DJ, Smith JE (1968) Filtration performance. In: Tranter DJ (ed) Zooplankton sampling. UNESCO monographs on oceanographic methodology, vol. 2. UNESCO Press, Paris, pp 27–56
Tucker MJ, Burton HR (1988) The inshore marine ecosystem off the Vestfold Hills, Antarctica. Hydrobiologia 165:129–139
van Franeker JA, Flores H, van Dorssen M (2009) The surface and under ice trawl (SUIT). In: Flores H (ed) Frozen desert alive—the role of sea ice for pelagic macrofauna and its predators. Dissertation, University of Groningen, pp 181–188. http://dissertations.ub.rug.nl/faculties/science/2009/h.flores/. Accessed 30 June 2015
Wiebe PH, Benfield MC (2003) From the Hensen net toward four-dimensional biological oceanography. Prog Oceanogr 56:7–136
Acknowledgments
This study was funded by the Italian National Programme for Antarctic Research (PNRA: Italian Programma Nazionale di Ricerche in Antartide) and has been carried out by the Project 2.b.3 “Ecology and Biogeochemistry of the Southern Ocean Matter and Energy Fluxes in the Cryosystems” (led by Prof. L. Guglielmo) in the framework of the PIPEX and PIED activities. Many thanks go to the technicians involved in the MicroNESS sampling procedures for their excellent cooperation during the fieldwork. Project development and manufacturing of the MicroNESS were carried out in cooperation with IdromarAmbiente Srl, Genoa, Italy (company website: www.idromarambiente.it). We are also indebted to three anonymous reviewers for their helpful suggestions that greatly improved the earlier version of the manuscript.
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Guglielmo, L., Arena, G., Brugnano, C. et al. MicroNESS: an innovative opening–closing multinet for under pack-ice zooplankton sampling. Polar Biol 38, 2035–2046 (2015). https://doi.org/10.1007/s00300-015-1763-3
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DOI: https://doi.org/10.1007/s00300-015-1763-3