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Diet: tissue stable isotope fractionation of carbon and nitrogen in blood plasma and whole blood of male reindeer Rangifer tarandus

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Abstract

Estimates of diet derived from stable isotope analyses are sensitive to the accuracy of corrections made for diet-tissue fractionation. In particular, diet-tissue fractionation in reindeer Rangifer tarandus may be expected to differ significantly from the generic values often used in stable isotope dietary calculations, given the known values obtained from other ungulates and the complex digestive system and nutrient recycling characteristic of the species. We fed domestic reindeer a homogenous artificial diet of known isotopic value in order to directly determine diet-tissue isotopic fractionation of carbon and nitrogen, the main elements used in stable isotope dietary analyses. Diet-tissue fractionation values for blood plasma were +3.5 ± 0.1‰ (δ13C) and +4.2 ± 0.3‰ (δ15N). Diet-tissue fractionation values for whole blood were +3.7 ± 0.2‰ (δ13C) and +2.5 ± 0.3‰ (δ15N). Metabolic turnover rates were clearly sufficient for complete tissue replacement over the period of artificial feeding for blood plasma, but may not have been so for whole blood, especially for δ15N. Our values, except for whole blood δ15N, differ considerably from the generic values often used in dietary studies and interspecific comparisons of trophic niche. The results underline the importance of obtaining as specific as possible fractionation values for the species, tissue, and in some cases sex and physiological status of animals under examination, and the potential problems associated with assuming ‘generic’ fractionation values when comparing species, especially where digestive processes are dissimilar.

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Acknowledgments

We would like to thank Agui Aiko for assistance with the mass spectrometry carried out for this research. Work was in part funded by the Norwegian Game Fund (Viltfondet) and by the Reindeer Industry Development Fund (Reindriftens utviklingsfond).

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Authors and Affiliations

  1. Norsk institutt for naturforskning, Tungasletta 2, 7485, Trondheim, Norway

    D. J. Halley & E. Gaare

  2. Section of Integrated Environmental Science, Faculty of Environmental Earth Science, Hokkaido University, N10W5 Kita-ku, Sapporo, Hokkaido, 060-0810, Japan

    M. Minagawa

  3. RKTL Porontutkimusasema, 99910, Kaamanen, Finland

    M. Nieminen

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  1. D. J. Halley
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  2. M. Minagawa
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  3. M. Nieminen
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  4. E. Gaare
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Correspondence to D. J. Halley.

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Halley, D.J., Minagawa, M., Nieminen, M. et al. Diet: tissue stable isotope fractionation of carbon and nitrogen in blood plasma and whole blood of male reindeer Rangifer tarandus . Polar Biol 33, 1303–1309 (2010). https://doi.org/10.1007/s00300-010-0817-9

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  • DOI: https://doi.org/10.1007/s00300-010-0817-9

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