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Plant Cell Reports DOI 10.1007/s00299-005-0048-7
Materials and methods, subsection Agrobacterium culture and infection medium, the fourth sentence should read:
On the day of infection, a bacterial pellet was obtained by spinning the overnight culture at 3500 rpm for 10 min and resuspended in infection medium containing 1/10 strength Gamborg's B5 salts (Gamborg et al. 1968), B5 vitamins, and 1/5 strength MSIII iron stock (Murashige and Skoog 1962), with 7.5 μM 6-benzylaminopurine (BAP), 0.7 μM gibberellic acid (GA3), 20 mM MES [2-(N-morpholino) ethanesulfonic acid], 3% sucrose and 200 μM acetosyringone, pH 5.4.
Materials and methods, subsection Explant preparation and infection, the seventh sentence should read:
The cocultivation medium is infection medium with 100−400 mg l−1 cysteine and 154 mg l−1 dithiothreitol, pH 5.4, and solidified with 0.425% Noble agar (Fisher Scientific, USA).
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The online version of the original article can be found at http://dx.doi.org/10.1007/s00299-005-0048-7
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Paz, M.M., Martinez, J.C., Kalvig, A.B. et al. Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation. Plant Cell Rep 25, 248 (2006). https://doi.org/10.1007/s00299-005-0113-2
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DOI: https://doi.org/10.1007/s00299-005-0113-2