Abstract
The role of extracellular chitinase in the biocontrol activity of Trichoderma virens was examined using genetically manipulated strains of this fungus. The T. virens strains in which the chitinase gene (cht42) was disrupted (KO) or constitutively over-expressed (COE) were constructed through genetic transformation. The resulting transformants were stable and showed patterns similar to the wild-type (WT) strain with respect to growth rate, sporulation, antibiotic production, colonization efficiency on cotton roots and growth/survival in soil. Biocontrol activity of the KO and COE strains were significantly decreased and enhanced, respectively against cotton seedling disease incited by Rhizoctonia solani when compared with the WT strain.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 4 September / 1 October 1998
Rights and permissions
About this article
Cite this article
Baek, JM., Howell, C. & Kenerley, C. The role of an extracellular chitinase from Trichoderma virens Gv29-8 in the biocontrol of Rhizoctonia solani. Curr Genet 35, 41–50 (1999). https://doi.org/10.1007/s002940050431
Issue Date:
DOI: https://doi.org/10.1007/s002940050431