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Preparation, characterization and in vitro biological study of silk fiber/methylcellulose composite for bone tissue engineering applications

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Abstract

In the present work, silk fiber (SF) and methylcellulose (MC) composites were fabricated by solvent casting method and characterized in detail. The interactions between SF/MC composites were studied in detail by Fourier transform infrared (FT-IR) spectroscopy and powder X-ray diffraction (XRD). The surface morphology and thermal stability were studied. Viscosity, thickness, folding endurance, tensile strength and antioxidant activity were analyzed for different ratios of SF/MC composite. Antimicrobial activity, in vitro biomimetic mineralization, hemocompatibility and cell viability of the SF/MC composite were studied. The deposition of calcium and phosphorus ions from simulated body fluid (SBF) onto SF/MC composite surface was evidenced from XRD, FT-IR and SEM–EDS. Inductively coupled plasma-optical emission spectrometry analysis (ICP-OES) was utilized to analyze leaching of Ca and P ions from the SBF. Hemolytic assay proves that the composites were compatible with blood and hemolytic ratio is found to be less than 5%. The MTT assay test against MG-63 suggests that the SF/MC composites are promising biomaterials for bone tissue engineering applications.

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Acknowledgements

We would like to thank VIT, Vellore, for providing all required facilities to carry out the work and IIT Madras for ICP-OES analysis.

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  1. Department of Chemistry, VIT University, Vellore, India

    Valarmathi Narayanan & Shanmugam Sumathi

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Correspondence to Shanmugam Sumathi.

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Narayanan, V., Sumathi, S. Preparation, characterization and in vitro biological study of silk fiber/methylcellulose composite for bone tissue engineering applications. Polym. Bull. 76, 2777–2800 (2019). https://doi.org/10.1007/s00289-018-2518-4

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