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MDR-TB Antibody Response (Western Blot) to Fractions of Isoniazid and Rifampicin Resistant Antigens of Mycobacterium tuberculosis

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Abstract

Drug-resistant TB poses a major threat to control of TB worldwide. Despite progress in the detection of Multidrug-resistant TB (MDR-TB) cases, a major diagnostic gap remains: 55 % of reported TB patients estimated to have MDR-TB were not detected in 2013. MDR-TB antigens were conjugated to CNBr-activated Sepharose 4B. Specific polyclonal antibodies against MDR-TB Ags were prepared in rabbits using two boosted injections of the MDR-TB antigen. The antibodies were purified and treated with susceptible TB to remove any non-specific and cross-reactive antibodies. In the present study, comparative analysis of electrophoretic pattern of different antigens of INH/RIF-resistant TB were studied for identifying protein profiles. A RIF-resistant TB antigen was shown here to have different protein profiles from INH-resistant TB isolate. The results of Western blotting analysis showed that in the RIF- and INH-resistant antigenic fractions some bands of 14.4 and 45 kDa as immunogenic were common. Moreover, four bands of RIF-resistant TB antigen fractions (16, 19, 21, and 45 KDa) and one band of INH-resistant TB (about 26 KDa) were detected as diagnostic antigens. This study suggests that the Western blot is an accurate test to survey INH- and RIF-resistant TB antigens of M. tuberculosis infection. These findings indicate that MDR-TB diagnosis (based on Ag detection) could be useful in the identification of disease stages that precede symptomatic and microbiologically positive TB, such as subclinical and incipient TB.

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Acknowledgments

We are sincerely grateful to the members of the Tuberculosis Department for the collection of TB strains. This study was part of the PhD fellowship projects (No. TP-9010) and funded by Pasteur Institute of Iran.

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Authors and Affiliations

  1. Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran

    Alireza Hadizadeh Tasbiti, Shamsi Yari, Mostafa Ghanei & Ahmadreza Bahrmand

  2. Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Mostafa Ghanei

  3. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

    Mohammad Ali Shokrgozar

Authors
  1. Alireza Hadizadeh Tasbiti
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  2. Shamsi Yari
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  3. Mostafa Ghanei
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  4. Mohammad Ali Shokrgozar
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  5. Ahmadreza Bahrmand
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Correspondence to Ahmadreza Bahrmand.

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Hadizadeh Tasbiti, A., Yari, S., Ghanei, M. et al. MDR-TB Antibody Response (Western Blot) to Fractions of Isoniazid and Rifampicin Resistant Antigens of Mycobacterium tuberculosis . Curr Microbiol 71, 638–642 (2015). https://doi.org/10.1007/s00284-015-0891-x

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  • DOI: https://doi.org/10.1007/s00284-015-0891-x

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