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Pelagibacterium nitratireducens sp.nov., A Marine Alphaproteobacterium Isolated from the East China Sea

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Abstract

A Gram-negative, rod-shaped, non-spore-forming aerobic bacterium, motile with a single polar flagellum, strain JLT2005T, was isolated from surface seawater collected from the East China Sea and formed ivory white colonies on a rich organic medium. The strain was positive for catalase, oxidase, and urease. It grew in the presence of 0–12 % (w/v) NaCl (optimum 5 %), at 20–35 °C (optimum 25 °C), or at pH 6–10 (optimum pH 9). The major fatty acids (>10 %) were C18:1ω7c, C19:0ω8c cyclo, C16:0, and C18:0. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and five unidentified glycolipids. Ubiquinone-10 and Ubiquinone-11 were present as the major quinones. The DNA G+C content was 74.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain JLT2005T belongs to the genus Pelagibacterium in the family Hyphomicrobiaceae, class Alphaproteobacteria. The closest neighbors were Pelagibacterium halotolerans B2T (98.7 % similarity) and Pelagibacterium luteolum 1_C16_27T (97.1 % similarity). DNA–DNA relatedness values of strain JLT2005T with P. halotolerans B2T and with P. luteolum 1_C16_27T were 31.6 and 25 %. Evidence from genotypic, chemotaxonomic, and phenotypic data shows that strain JLT2005T represents a novel species of the genus Pelagibacterium, for which the name Pelagibacterium nitratireducens sp. nov is proposed. The type strain is JLT2005T (=CGMCC 1.10829T =JCM 17767T).

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Acknowledgments

The authors would like to thank Dr. Yanan Wang, Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences; Dr. Xiaojun Yan, School of Life Science & Biological Engineering, Ningbo University; Dr. Henglin Cui, School of Food & Biological Engineering, Jiangsu University; and Dr. Li Gu, Third Institute of Oceanography, State Oceanic Administration for their valuable assistance. Professor John Hodgkiss is also thanked for his assistance with English. This work was supported by 973 program (2011CB808800), SOA project (201105021), MOST project (2012AA092003), and NSFC (41191021). Dr. Rui Zhang was also supported by NSFC (40906059).

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Authors and Affiliations

  1. State Key Laboratory for Marine Environmental Science, Xiamen University, Xiamen, 361005, People’s Republic of China

    Qipei Li, Yongle Xu, Keshao Liu, Lanlan Cai, Yingnan Fu, Jia Sun & Rui Zhang

  2. Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen, 361005, People’s Republic of China

    Qipei Li, Yongle Xu, Keshao Liu, Lanlan Cai, Yingnan Fu, Jia Sun & Rui Zhang

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  1. Qipei Li
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  2. Yongle Xu
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  7. Rui Zhang
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Correspondence to Rui Zhang.

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Li, Q., Xu, Y., Liu, K. et al. Pelagibacterium nitratireducens sp.nov., A Marine Alphaproteobacterium Isolated from the East China Sea. Curr Microbiol 66, 450–455 (2013). https://doi.org/10.1007/s00284-012-0299-9

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  • DOI: https://doi.org/10.1007/s00284-012-0299-9

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