Abstract
The concept of utilizing putative and unique gene sequences for the design of species specific probes was tested. The abundance profile of assigned functions within the Lactobacillus plantarum genome was used for the identification of the putative and unique gene sequence, csh. The targeted gene (csh) was used as the template for PCR amplification and construction of a non-radioactive DIG labeled probe. The csh derived probe aided in the preliminary and rapid identification of L. plantarum from mixed cultures by colony hybridization. The method described here for the rapid identification of L. plantarum can also be applied for the rapid detection of other bacteria if a unique gene sequence can be identified from its complete genome sequence.
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The author thanks Ms. Ashlee Webber at the USDA-ARS, SAA Food Science Research Unit located in Raleigh, NC for technical assistance and Ms. Sandra Parker for excellent secretarial assistance.
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Pérez-Díaz, I.M. Putative and Unique Gene Sequence Utilization for the Design of Species Specific Probes as Modeled by Lactobacillus plantarum . Curr Microbiol 66, 266–270 (2013). https://doi.org/10.1007/s00284-012-0265-6
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DOI: https://doi.org/10.1007/s00284-012-0265-6