Abstract
The aim of this study was to identify novel biofilm inhibitors from actinomycetes isolated from the Arctic against Vibrio cholerae, the causative agent of cholera. The biofilm inhibitory activity of actinomycetes was assessed using biofilm assay and was confirmed using air–liquid interphase coverslip assay. The potential isolates were identified using 16S rRNA gene sequencing. Of all, three isolates showed significant biofilm inhibition against V. cholerae. The results showed that 20% of the actinomycetes culture supernatant could inhibit up to 80% of the biofilm formation. When different extracted fractions were assessed, significant biofilm inhibition activity was only seen in the diethyl ether fraction of A745. At 200 μg ml−1 of diethyl ether fraction, 60% inhibition of V. cholerae biofilm was observed. The two potential isolates were found to be Streptomyces sp. and one isolate belonged to Nocardiopsis sp. This is the first report showing a Streptomyces sp. and Nocardiopsis sp. isolated from the Arctic having a biofilm inhibitory activity against V. cholerae. The spread of drug resistant V. cholerae strains is a major clinical problem and the ineffectiveness in antibiotic treatment necessitates finding new modes of prevention and containment of the disease, cholera. The formation of biofilms during the proliferation of V. cholerae is linked to its pathogenesis. Hence, the bioactive compound from the culture supernatant of the isolates identified in this study may be a promising source for the development of a potential quorum sensing inhibitors against V. cholerae.
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Acknowledgments
This study was supported by an intramural support from Rajiv Gandhi Centre for Biotechnology, Trivandrum, India. Dr. Sabu Thomas is thankful to Shri. Rasik Ravindra, Director and Dr. S. Rajan, Programme Director, National Centre for Antarctic and Ocean Research, Goa and Ministry of Earth Science, Govt. of India for having provided all the facilities and support during the Indian Arctic Expedition-2009. The authors are thankful to Prof. M. Radhakrishna Pillai, Director, RGCB for the facilities provided.
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Augustine, N., Peter A, W., Kerkar, S. et al. Arctic Actinomycetes as Potential Inhibitors of Vibrio cholerae Biofilm. Curr Microbiol 64, 338–342 (2012). https://doi.org/10.1007/s00284-011-0073-4
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DOI: https://doi.org/10.1007/s00284-011-0073-4