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A Low-Cost Fermentation Medium for Thermophilic Protease Production by Streptomyces sp. 594 Using Feather Meal and Corn Steep Liquor

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Abstract

Protease production by Streptomyces sp. 594 was obtained after submerged fermentation (SF) and solid-state fermentation (SSF) using feather meal (FM) and corn steep liquor (CSL) as sole sources of carbon and nitrogen. Enzyme productions were 13.4 U ml−1 in SF and 21.5 U g−1 in SSF; these values were approximately 86% and 39% higher, respectively, than those obtained previously when yeast extract was used in place of CSL. The proteases, which belong to the serine and metalloproteinase classes, were active at high temperatures (55°C to 90°C) and over a wide range of pH values (5.0 to 10.0). Thus, these thermophilic proteases have shown interesting properties for industrial purposes. As far as we are concerned, this is the first contribution toward the microbial production of thermophilic proteases by a streptomycete using a low-cost medium composed of industrial poultry (FM) and corn processing by-products (CSL).

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Acknowledgments

Financial support from FAPERJ and CNPq is gratefully acknowledged.

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Authors and Affiliations

  1. Instituto de Microbiologia Professor Paulo de Góes, Bloco I, Departamento de Microbiologia Geral, Laboratory 51, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ, Brazil, CEP 21.941−590

    L. A. I. De Azeredo & R. R. R. Coelho

  2. Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca 94, Centro, Rio de Janeiro, RJ, Brazil, CEP 20.211-040

    M. B. De Lima

  3. Instituto de Química, Departamento de Bioquímica, Laboratory 549-2, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ, Brazil, CEP 21.945-970

    D. M. G. Freire

Authors
  1. L. A. I. De Azeredo
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  2. M. B. De Lima
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  3. R. R. R. Coelho
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  4. D. M. G. Freire
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Correspondence to D. M. G. Freire.

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De Azeredo, L.A.I., De Lima, M.B., Coelho, R.R.R. et al. A Low-Cost Fermentation Medium for Thermophilic Protease Production by Streptomyces sp. 594 Using Feather Meal and Corn Steep Liquor. Curr Microbiol 53, 335–339 (2006). https://doi.org/10.1007/s00284-006-0163-x

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  • DOI: https://doi.org/10.1007/s00284-006-0163-x

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