Abstract
Objective
The aim of the present study was to show the feasibility and describe the first results of a 3D reconstruction of the venous network of the lower limbs in human fetus using the computer-assisted anatomical dissection (CAAD) technique.
Materials and methods
We used limbs from two human fetuses, respectively, 14 and 15 weeks gestation old. Specimens were fixed in 10 % formalin, embedded in paraffin wax and serially sectioned at 10 m. The histological slices were stained using HES and Masson Trichrome for soft tissues identification. Immunolabeling techniques using the Protein S-100 marker and the D2-40 marker were used to identify nerves and vessels, respectively. Stained slices were aligned manually, labeled and digitalized. The segmentation of all anatomical structures was achieved using the WinSurf® software after manual drawing.
Results
A 3D interactive vectorial model of the whole leg, including skin, bone, muscles, arteries, veins, and nerves was obtained. In all limbs, we observed the presence of a big axial vein traveling along the sciatic nerve. In addition, the femoral vein appeared as a small plexus. Although this is a common anatomical feature at the end of organogenesis, this feature is observed in only 9 % of adults. Usually interpreted as an “anatomical variation of the femoral vein” it should be considered as a light truncular malformation. These observations bring further support to our proposed “angio-guiding nerves” hypothesis.
Conclusion
This preliminary study shows that the CAAD technique provided an accurate 3D reconstruction of the fetal leg veins anatomy. It should bring a new insight for the understanding of the different steps of development of the human venous system.
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Acknowledgments
Thanks to the students of the Master of clinical Anatomy of the Paris Descartes University who participated to this work: Laura Gouzien, Laeticia Msika, Rodolphe Bihannic, Vincent Front, and Paul Morin.
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Kurobe, N., Hakkakian, L., Chahim, M. et al. Three-dimensional reconstruction of the lower limb’s venous system in human fetuses using the computer-assisted anatomical dissection (CAAD) technique. Surg Radiol Anat 37, 231–238 (2015). https://doi.org/10.1007/s00276-014-1350-2
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DOI: https://doi.org/10.1007/s00276-014-1350-2