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TANTIGEN: a comprehensive database of tumor T cell antigens

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Abstract

Tumor T cell antigens are both diagnostically and therapeutically valuable molecules. A large number of new peptides are examined as potential tumor epitopes each year, yet there is no infrastructure for storing and accessing the results of these experiments. We have retroactively cataloged more than 1000 tumor peptides from 368 different proteins, and implemented a web-accessible infrastructure for storing and accessing these experimental results. All peptides in TANTIGEN are labeled as one of the four categories: (1) peptides measured in vitro to bind the HLA, but not reported to elicit either in vivo or in vitro T cell response, (2) peptides found to bind the HLA and to elicit an in vitro T cell response, (3) peptides shown to elicit in vivo tumor rejection, and (4) peptides processed and naturally presented as defined by physical detection. In addition to T cell response, we also annotate peptides that are naturally processed HLA binders, e.g., peptides eluted from HLA in mass spectrometry studies. TANTIGEN provides a rich data resource for tumor-associated epitope and neoepitope discovery studies and is freely available at http://cvc.dfci.harvard.edu/tantigen/ or http://projects.met-hilab.org/tadb (mirror).

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Abbreviations

BLAST:

Basic local alignment search tool

COSMIC:

The catalogue of somatic mutations in cancer

NCBI:

National Center for Biotechnology Information

ORF:

Open reading frame

TAs:

Tumor antigens

TSAs:

Tumor-specific antigens

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Acknowledgements

This work was supported by The Danish Council for Independent Research Grant 4184-00211B (Lars Rønn Olsen), NIH Grant UO1 AI090043 and SU2C-AACR-DT13-14 (Ellis Reinherz) and Dana-Farber Cancer Institute, Cancer Vaccine Center Funds (Guang Lan Zhang and Vladimir Brusic).

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    Authors and Affiliations

    1. Cancer Vaccine Center, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA, 02115, USA

      Lars Rønn Olsen, Songsak Tongchusak, Honghuang Lin, Ellis L. Reinherz, Vladimir Brusic & Guang Lan Zhang

    2. Department of Bio and Health Informatics, Technical University of Denmark, Lyngby, 2800, Denmark

      Lars Rønn Olsen

    3. Section of Computational Biomedicine, Department of Medicine, Boston University School of Medicine, 72 E. Concord Street, B-616, Boston, MA, 02118, USA

      Honghuang Lin

    4. Department of Medicine, Harvard Medical School, 25 Shattuck Street, Boston, MA, 02115, USA

      Ellis L. Reinherz

    5. Laboratory of Immunobiology, Dana-Farber Cancer Institute, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA

      Ellis L. Reinherz

    6. School of Medicine and Bioinformatics Center, Nazarbayev University, Astana, Kazakhstan

      Vladimir Brusic

    7. Department of Computer Science, Metropolitan College, Boston University, Room 254808 Commonwealth Ave, Boston, MA, 02215, USA

      Vladimir Brusic & Guang Lan Zhang

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    1. Lars Rønn Olsen
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    Correspondence to Guang Lan Zhang.

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    Lars Rønn Olsen and Songsak Tongchusak contributed equally to the work.

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    Olsen, L.R., Tongchusak, S., Lin, H. et al. TANTIGEN: a comprehensive database of tumor T cell antigens. Cancer Immunol Immunother 66, 731–735 (2017). https://doi.org/10.1007/s00262-017-1978-y

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    • DOI: https://doi.org/10.1007/s00262-017-1978-y

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