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Comparative analysis of lignin peroxidase and manganese peroxidase activity on coniferous and deciduous wood using ToF-SIMS

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Abstract

White-rot fungi are distinguished by their ability to efficiently degrade lignin via lignin-modifying type II peroxidases, including manganese peroxidase (MnP) and lignin peroxidase (LiP). In the present study, time-of flight secondary ion mass spectrometry (ToF-SIMS) was used to evaluate lignin modification in three coniferous and three deciduous wood preparations following treatment with commercial preparations of LiP and MnP from two different white-rot fungi. Percent modification of lignin was calculated as a loss of intact methoxylated lignin over nonfunctionalized aromatic rings, which is consistent with oxidative cleavage of methoxy moieties within the lignin structure. Exposure to MnP resulted in greater modification of lignin in coniferous compared to deciduous wood (28 vs. 18 % modification of lignin); and greater modification of G-lignin compared to S-lignin within the deciduous wood samples (21 vs. 12 %). In contrast, exposure to LiP resulted in similar percent modification of lignin in all wood samples (21 vs 22 %), and of G- and S-lignin within the deciduous wood (22 vs. 23 %). These findings suggest that the selected MnP and LiP may particularly benefit delignification of coniferous and deciduous wood, respectively. Moreover, the current analysis further demonstrates the utility of ToF-SIMS for characterizing enzymatic modification of lignin in wood fibre along with potential advantages over UV and HPCL-MS detection of solubilized delignification products.

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Authors and Affiliations

  1. Department of Chemical Engineering & Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, M5S 3E5, Canada

    Jacqueline MacDonald, Robyn E. Goacher & Emma R. Master

  2. London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, N5V 4T3, Canada

    Jacqueline MacDonald

  3. Department of Biochemistry, Chemistry and Physics, Niagara University, Niagara, NY, USA

    Robyn E. Goacher

  4. Great Lakes Forestry Centre, 1219 Queen Street East, Sault Ste. Marie, ON, P6A 2E5, Canada

    Mamdouh Abou-Zaid

  5. Department of Chemical and Biochemical Engineering, Western University, Claudette Mackay-Lassonde Pavilion Room CMLP 2329, London, ON, N6A 5B9, Canada

    Mamdouh Abou-Zaid

Authors
  1. Jacqueline MacDonald
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  2. Robyn E. Goacher
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  3. Mamdouh Abou-Zaid
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  4. Emma R. Master
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Correspondence to Emma R. Master.

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This work was supported by grants from the Natural Sciences and Engineering Research Council and by the Government of Canada through Genome Canada and the Ontario Genomics Institute.

(Grant 2009-OGI-ABC-1405), as part of the Bioproducts and Enzymes from Environmental Metagenomes (BEEM) Project. The TOF-SIMS analyses were performed at Surface.

Interface Ontario, an analytical facility funded by the Canadian Foundation for Innovation (project no.11128), the Ontario Research Fund and institutional partners.

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The authors declare that they have no competing interests.

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MacDonald, J., Goacher, R.E., Abou-Zaid, M. et al. Comparative analysis of lignin peroxidase and manganese peroxidase activity on coniferous and deciduous wood using ToF-SIMS. Appl Microbiol Biotechnol 100, 8013–8020 (2016). https://doi.org/10.1007/s00253-016-7560-2

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