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Inoculum pre-treatment affects the fermentative activity of hydrogen-producing communities in the presence of 5-hydroxymethylfurfural

  • Bioenergy and biofuels
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Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

To enhance the productivity of mixed microbial cultures for fermentative bio-hydrogen production, chemical-physical pre-treatments of the original seed are needed to suppress the activity of hydrogen (H2)-consuming microbes. This approach might influence negatively the composition and diversity of the hydrogen-producing community with consequences on the functional stability of the H2-producing systems in case of perturbations. In this study, we aimed at investigating the effect of different types of pre-treatment on the performance of hydrogen production systems in the presence of an inhibitor, such as 5-hydroxymethylfurfural (HMF). The efficiency and the microbial community structure of batch reactors amended with HMF and inoculated with non-pretreated and pretreated (acid, heat shock, and aeration) anaerobic sludge were evaluated and compared with control systems. The type of pre-treatments influenced the microbial community assembly and activity in inhibited systems, with significant effect on the performance. Cumulative H2 production tests showed that the pre-aerated systems (control and HMF inhibited) were the most efficient, while the difference of the lag phase of the pre-acidified control and HMF-added test was negligible. Analyses of the structure of the enriched microbial community in the systems through PCR-denaturing gradient gel electrophoresis (DGGE) followed by band sequencing revealed that the differences in performance were mostly related to shifts in the metabolic pathways rather than in the predominant species. In conclusion, the findings suggest that the use of specific inoculum pre-treatment could contribute to regulate the metabolic activity of the fermentative H2-producing bacteria in order to enhance the bio-energy production.

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Acknowledgments

The authors would like to thank Dr. Mélanie Pierra for her helpful advice and discussion and Salvatore Burbaci for his laboratory support.

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Conflict of interest

All the authors declare that they no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Funding

This project was funded by the European Commission, Directorate-General for Research & Innovation, SP4 (Capacities, Coordination and Support Action), Seventh Framework Programme (FP7); Grant Agreement No. 286269.

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Authors and Affiliations

  1. Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università degli Studi di Foggia, Via Napoli 25, Foggia, Italy

    Micol Bellucci, Giuseppe Botticella & Luciano Beneduce

  2. STAR Agroenergy Research Group, University of Foggia, Via Gramsci, 89-91, Foggia, Italy

    Micol Bellucci, Matteo Francavilla & Luciano Beneduce

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  1. Micol Bellucci
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  2. Giuseppe Botticella
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  3. Matteo Francavilla
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  4. Luciano Beneduce
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Correspondence to Luciano Beneduce.

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Bellucci, M., Botticella, G., Francavilla, M. et al. Inoculum pre-treatment affects the fermentative activity of hydrogen-producing communities in the presence of 5-hydroxymethylfurfural. Appl Microbiol Biotechnol 100, 493–504 (2016). https://doi.org/10.1007/s00253-015-7002-6

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  • DOI: https://doi.org/10.1007/s00253-015-7002-6

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