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Novel cyclohexane monooxygenase from Acidovorax sp. CHX100

  • Environmental biotechnology
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Abstract

Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols.

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Acknowledgments

We would like to thank Dr. Josef Altenbuchner (Institute of Industrial Genetics—University of Stuttgart) for kindly providing the strain E. coli S17.1 as well as the pCro2a transposon mutagenesis vector.

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Author notes

  1. Diego Salamanca

    Present address: Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany

Authors and Affiliations

  1. Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany

    Diego Salamanca & Daniel Dobslaw

  2. Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany

    Rohan Karande & Andreas Schmid

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  1. Diego Salamanca
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  2. Rohan Karande
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  4. Daniel Dobslaw
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Correspondence to Daniel Dobslaw.

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Salamanca, D., Karande, R., Schmid, A. et al. Novel cyclohexane monooxygenase from Acidovorax sp. CHX100. Appl Microbiol Biotechnol 99, 6889–6897 (2015). https://doi.org/10.1007/s00253-015-6599-9

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  • DOI: https://doi.org/10.1007/s00253-015-6599-9

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