Abstract
Small RNAs, a large class of ancient posttranscriptional regulators, have recently attracted considerable attention. A plethora of small RNAs has been identified and characterized, many of which belong to the major small noncoding RNA (sRNA) or riboswitch families. It has become increasingly clear that most small RNAs play critical regulatory roles in many processes and are, therefore, considered to be powerful tools for metabolic engineering and synthetic biology. In this review, we describe recent achievements in the identification, characterization, and application of small RNAs. We give particular attention to advances in the design and synthesis of novel sRNAs and riboswitches for metabolic engineering. In addition, a novel strategy for hierarchical control of global metabolic pathways is proposed.
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This work was financially supported by the Major State Basic Research Development Program of China (973 Program, 2012CB720802, 2014CB745103, 2013CB733902, 2013CB733602), the National Natural Science Foundation of China (31200020, 31130043), the National High Technology Research and Development Program of China (863 Program, 2011AA100905), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1135), the National Science Foundation for Post-doctoral Scientists of China (2013M540414), the Jiangsu Planned Projects for Postdoctoral Research Funds (1301010B), and the 111 Project.
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Kang, Z., Zhang, C., Zhang, J. et al. Small RNA regulators in bacteria: powerful tools for metabolic engineering and synthetic biology. Appl Microbiol Biotechnol 98, 3413–3424 (2014). https://doi.org/10.1007/s00253-014-5569-y
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DOI: https://doi.org/10.1007/s00253-014-5569-y