Applied Microbiology and Biotechnology

, Volume 98, Issue 8, pp 3413–3424

Small RNA regulators in bacteria: powerful tools for metabolic engineering and synthetic biology

Authors

    • Synergetic Innovation Center of Food Safety and Nutrition
    • The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan University
    • Synergy Innovation Center of Modern Industrial Fermentation
    • Yixing Union Biochemical Co., Ltd.
  • Chuanzhi Zhang
    • The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan University
  • Junli Zhang
    • The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan University
  • Peng Jin
    • The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan University
  • Juan Zhang
    • Synergetic Innovation Center of Food Safety and Nutrition
    • The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan University
    • Synergy Innovation Center of Modern Industrial Fermentation
  • Guocheng Du
    • Synergetic Innovation Center of Food Safety and Nutrition
    • The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan University
    • The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of EducationJiangnan University
    • Synergy Innovation Center of Modern Industrial Fermentation
    • Synergetic Innovation Center of Food Safety and Nutrition
    • The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan University
    • Synergy Innovation Center of Modern Industrial Fermentation
    • National Engineering Laboratory for Cereal Fermentation TechnologyJiangnan University
Mini-Review

DOI: 10.1007/s00253-014-5569-y

Cite this article as:
Kang, Z., Zhang, C., Zhang, J. et al. Appl Microbiol Biotechnol (2014) 98: 3413. doi:10.1007/s00253-014-5569-y

Abstract

Small RNAs, a large class of ancient posttranscriptional regulators, have recently attracted considerable attention. A plethora of small RNAs has been identified and characterized, many of which belong to the major small noncoding RNA (sRNA) or riboswitch families. It has become increasingly clear that most small RNAs play critical regulatory roles in many processes and are, therefore, considered to be powerful tools for metabolic engineering and synthetic biology. In this review, we describe recent achievements in the identification, characterization, and application of small RNAs. We give particular attention to advances in the design and synthesis of novel sRNAs and riboswitches for metabolic engineering. In addition, a novel strategy for hierarchical control of global metabolic pathways is proposed.

Keywords

Small RNARiboswitchGene expressionPosttranscriptional regulationMetabolic engineeringSynthetic biology

Copyright information

© Springer-Verlag Berlin Heidelberg 2014