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Response of Saccharomyces cerevisiae to D-limonene-induced oxidative stress

  • Applied microbial and cell physiology
  • Published:
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Abstract

In the present study, we investigated the mode of cell response induced by D-limonene in Saccharomyces cerevisiae. D-limonene treatment was found to be accompanied by intracellular accumulation of reactive oxygen species (ROS). Since ROS impair cell membranes, an engineered strain with enhanced membrane biosynthesis exhibited a higher tolerance to D-limonene. Subsequent addition of an ROS scavenger significantly reduced the ROS level and alleviated cell growth inhibition. Thus, D-limonene-induced ROS accumulation plays an important role in cell death in S. cerevisiae. In D-limonene-treated S. cerevisiae strains, higher levels of antioxidants, antioxidant enzymes, and nicotinamide adenine dinucleotide phosphate (NADPH) were synthesized. Quantitative real-time PCR results also verified that D-limonene treatment triggered upregulation of genes involved in the antioxidant system and the regeneration of NADPH at the transcription level in S. cerevisiae. These data indicate that D-limonene treatment results in intracellular ROS accumulation, an important factor in cell death, and several antioxidant mechanisms in S. cerevisiae were enhanced in response to D-limonene treatment.

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Acknowledgments

This work was supported by the Major State Basic Research Development Program of China (973 Program, 2012CB720802), the National Natural Science Foundation of China (31000807, 31130043, 21276109), the Natural Science Foundation of Jiangsu Province (BK2010150, BK2011004), New Century Excellent Talents in University (NCET-12-0876), the Foundation for the Author of National Excellent Doctoral Dissertation of PR China (FANEDD, 2011046), the Fundamental Research Funds for the Central Universities (JUSRP51307A) and the Open Project Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (KLIB-KF200907).

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China

    Jidong Liu, Yibo Zhu, Guocheng Du, Jingwen Zhou & Jian Chen

  2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China

    Guocheng Du & Jian Chen

  3. School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China

    Jingwen Zhou & Jian Chen

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  1. Jidong Liu
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  2. Yibo Zhu
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  3. Guocheng Du
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  4. Jingwen Zhou
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  5. Jian Chen
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Correspondence to Jingwen Zhou or Jian Chen.

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Liu, J., Zhu, Y., Du, G. et al. Response of Saccharomyces cerevisiae to D-limonene-induced oxidative stress. Appl Microbiol Biotechnol 97, 6467–6475 (2013). https://doi.org/10.1007/s00253-013-4931-9

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