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Kinetic analysis of decreased sperm fertilizing ability by fluorides and fluoroaluminates: a tool for analyzing the effect of environmental substances on biological events

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Abstract

Fluorides and fluoroaluminates decrease mouse sperm fertilizing potential by modifying the process of sperm preparation for fertilization, so-called capacitation, followed by acrosome reaction (AR). Capacitation was monitored by protein tyrosine phosphorylation (pTyr), and AR was induced consequently. The aim of this study was to apply kinetic analysis to the previously obtained dependences of pTyr and AR at capacitation times, and propose a mathematical theory for a mechanism when sperm maturation ability is amended by external stimuli. The experimental input data, previously obtained, are consistent with the proposed theory and the results of kinetic analysis show that sperm capacitation runs as two subsequent first-order steps. Firstly, an unstable intermediate is formed and then gradually decomposes. The time corresponding to the maximal production of the unstable intermediate is probably most suitable for sperm obtaining the ability to fertilize the egg. The presented calculations indicate that the application of kinetic analysis can serve as a tool to predict or confirm a course of biological events that are modified by external factors, and therefore the proposed theory shall be of interest to a broad scientific audience.

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Acknowledgments

This work was supported by the project “BIOCEV—Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University” (CZ.1.05/1.1.00/02.0109), from the European Regional Development Fund (www.biocev.eu), by the Grant Agency of the Czech Republic No. P502-14-05547S, and by the Institutional support of the Institute of Biotechnology RVO: 86652036.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, 128 43, Prague 2, Czech Republic

    Zuzana Bosakova, Antonin Tockstein, Hana Adamusova & Pavel Coufal

  2. Department of Cell Biology, Faculty of Science, Charles University, Vinicna 7, 128 44, Prague 2, Czech Republic

    Natasa Sebkova

  3. Biocev Group, Department of Zoology, Faculty of Science, Charles University, Vinicna 7, 128 44, Prague 2, Czech Republic

    Katerina Dvorakova-Hortova

  4. Laboratory of Reproductive Biology, Institute of Biotechnology AS CR, v.v.i., Videnska 1083, 14220, Prague 4, Czech Republic

    Natasa Sebkova & Katerina Dvorakova-Hortova

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  1. Zuzana Bosakova
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  2. Antonin Tockstein
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  3. Hana Adamusova
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  4. Pavel Coufal
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  5. Natasa Sebkova
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  6. Katerina Dvorakova-Hortova
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Correspondence to Zuzana Bosakova or Katerina Dvorakova-Hortova.

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All authors of this manuscript (Zuzana Bosakova, Antonin Tockstein, Hana Adamusova, Pavel Coufal, Natasa Sebkova, and Katerina Dvorakova-Hortova) declare that no competing financial interests exist.

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Bosakova, Z., Tockstein, A., Adamusova, H. et al. Kinetic analysis of decreased sperm fertilizing ability by fluorides and fluoroaluminates: a tool for analyzing the effect of environmental substances on biological events. Eur Biophys J 45, 71–79 (2016). https://doi.org/10.1007/s00249-015-1078-x

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  • DOI: https://doi.org/10.1007/s00249-015-1078-x

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