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Characterizing the Adherence Profiles of Virulent Vibrio parahaemolyticus Isolates

  • Environmental Microbiology
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Abstract

The human pathogen Vibrio parahaemolyticus is a leading cause of seafood-borne illness in the USA, and infections with V. parahaemolyticus typically result from eating raw or undercooked oysters. V. parahaemolyticus has been shown to be highly resistant to oyster depuration, suggesting that the bacterium possesses specific mechanisms or factors for colonizing oysters and persisting during depuration. In this study, we characterized eight different V. parahaemolyticus strains for differences in resistance to oyster depuration, biofilm formation, and motility. While each strain exhibited distinct phenotypes in the various assays, we determined that biofilm formation on abiotic surfaces, such as glass or plastic, does not directly correlate with bacterial retention in oysters during depuration. However, we did observe that the motility phenotype of a strain appeared to be a better indicator for persistence in the oyster. Further studies examining the molecular mechanisms underlying the observed colonization differences by these and other V. parahaemolyticus strains may provide beneficial insights into what critical factors are required for proficient colonization of the Pacific oyster.

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Acknowledgements

We would like to thank Dr. Linda McCarter for kindly providing the opaque, translucent, and non-motile V. parahaemolyticus strains and the Oregon Oyster Farm (Newport, OR) for providing the animals used in this study. This study was funded by the National Research Initiative Food Safety and Epidemiology Program (32.0A) Grant No. 2008–35201–04580 and the Agriculture and Food Research Initiative Grant No. 2011-68003-30005 of the USDA National Institute of Food and Agriculture.

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Authors and Affiliations

  1. Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, 97331, USA

    Alisha M. Aagesen & Claudia C. Häse

  2. Seafood Research and Education Center, Oregon State University, Astoria, OR, 97103, USA

    Sureerat Phuvasate & Yi-Cheng Su

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  1. Alisha M. Aagesen
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  2. Sureerat Phuvasate
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  3. Yi-Cheng Su
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  4. Claudia C. Häse
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Correspondence to Claudia C. Häse.

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Dr. Yi-Cheng Su passed away December 5, 2016.

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Aagesen, A.M., Phuvasate, S., Su, YC. et al. Characterizing the Adherence Profiles of Virulent Vibrio parahaemolyticus Isolates. Microb Ecol 75, 152–162 (2018). https://doi.org/10.1007/s00248-017-1025-8

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