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Nandrolone Decanoate Induces Genetic Damage in Multiple Organs of Rats

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Abstract

To evaluate the impact potential of nandrolone decanoate on DNA damage in multiple organs of Wistar rats by means of single-cell gel (comet) assay and micronucleus test. A total of 15 animals were distributed into three groups of five animals each as follows: control group = animal not exposed to nandrolone decanoate; experimental group = animals exposed to nandrolone decanoate for 24 h at 5 mg/kg subcutaneously; and experimental group = animals exposed to nandrolone decanoate for 24 h at 15 mg/kg subcutaneously. Significant statistical differences (p < 0.05) were noted in peripheral blood, liver, and heart cells exposed to nandrolone decanoate at the two doses evaluated. A clear dose–response relationship was observed between groups. Kidney cells showed genetic damage at only the highest dose (15 mg/kg) used. However, micronucleus data did not show remarkable differences among groups. In conclusion, the present study indicates that nandrolone decanoate induces genetic damage in rat blood, liver, heart, and kidney cells as shown by single-cell gel (comet) assay results.

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Acknowledgments

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnologico and CAPES. D. A. R. is the recipient of fellowship from CNPq.

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Authors and Affiliations

  1. Department of Biosciences, Federal University of São Paulo, São Paulo, 11060-001, Brazil

    Renan Pozzi, Kelly Rosseti Fernandes, Ana Claudia Muniz Renno & Daniel Araki Ribeiro

  2. Department of Pathology, Federal University of São Paulo, São Paulo, 11060-001, Brazil

    Carolina Foot Gomes de Moura & Daniel Araki Ribeiro

  3. Postgraduate Program in Rehabilitation Sciences, Nove de Julho Unversity, São Paulo, Brazil

    Raquel Agnelli Mesquita Ferrari & Kristianne Porta Santos Fernandes

  4. Departamento de Biociências, Universidade Federal de São Paulo-UNIFESP, Av. Ana Costa, 95, Vila Mathias, Santos, SP, 11060-001, Brazil

    Daniel Araki Ribeiro

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  1. Renan Pozzi
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  2. Kelly Rosseti Fernandes
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  3. Carolina Foot Gomes de Moura
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  4. Raquel Agnelli Mesquita Ferrari
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  5. Kristianne Porta Santos Fernandes
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  6. Ana Claudia Muniz Renno
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  7. Daniel Araki Ribeiro
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Correspondence to Daniel Araki Ribeiro.

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Pozzi, R., Fernandes, K.R., de Moura, C.F.G. et al. Nandrolone Decanoate Induces Genetic Damage in Multiple Organs of Rats. Arch Environ Contam Toxicol 64, 514–518 (2013). https://doi.org/10.1007/s00244-012-9848-2

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  • DOI: https://doi.org/10.1007/s00244-012-9848-2

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