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Biomolecular Interactions of Tannin Isolated from Oenothera gigas with Liposomes

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Abstract

We have examined the interaction between hydrolysable tannin 1-O-galloyl-4,6-hexahydroxydiphenoyl-β-d-glucose (OGβDG) with neutral liposomes as a model of cell membranes composed of three lipids: lecithin, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at different mass ratios. OGβDG in the concentration range 0.5–15 µg/ml (0.4–12 µM) strongly interacts with liposomal membranes by changing their structure, surface charge and fluidity. Used OGβDG molecules decrease and increase the rigidity of hydrophilic surface and hydrophobic parts of liposomes, respectively. At higher concentrations of tannin (>15 µM), liposomes are aggregated. Fourier Transform Infra-Red (FTIR) analysis showed that mainly –OH groups from OGβDG and also PO2− groups from phospholipids are responsible for the interaction. Obtained data indicate the importance of membrane lipid composition in interactions between tannins and cells.

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Authors and Affiliations

  1. Department of Biophysics, Laboratory of Molecular Biophysics, Faculty of Biology and Chemistry, University of Bialystok, 15-950, Bialystok, Poland

    Szymon Sekowski & Maria Zamaraeva

  2. Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236, Lodz, Poland

    Maksim Ionov & Maria Bryszewska

  3. Department of Natural Products Chemistry, Faculty of Biology and Chemistry, University of Bialystok, 15-950, Bialystok, Poland

    Alina Dubis

  4. Bio-Nano-Techno Center, Faculty of Biology and Chemistry, University of Bialystok, 15-950, Bialystok, Poland

    Alina Dubis

  5. Institute of Bioorganic Chemistry, Academy of Science of Republic of Uzbekistan, Tashkent-143, Uzbekistan

    Saidmukhtar Mavlyanov

Authors
  1. Szymon Sekowski
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  2. Maksim Ionov
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  3. Alina Dubis
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  4. Saidmukhtar Mavlyanov
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  5. Maria Bryszewska
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  6. Maria Zamaraeva
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Correspondence to Szymon Sekowski.

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Sekowski, S., Ionov, M., Dubis, A. et al. Biomolecular Interactions of Tannin Isolated from Oenothera gigas with Liposomes. J Membrane Biol 249, 171–179 (2016). https://doi.org/10.1007/s00232-015-9858-x

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  • DOI: https://doi.org/10.1007/s00232-015-9858-x

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