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Involvement of Aquaporin-5 Water Channel in Osmoregulation in Parotid Secretory Granules

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Abstract

Aquaporins (AQPs) are a family of channel proteins that allow water or very small solutes to pass, functioning in tissues where the rapid and regulated transport of fluid is necessary, such as the kidney, lung, and salivary glands. Aquaporin-5 (AQP5) has been demonstrated to localize on the luminal surface of the acinar cells of the salivary glands. In this paper, we investigated the expression and function of AQP5 in the secretory granules of the rat parotid gland. AQP5 was detected in the secretory granule membranes by immunoblot analysis. The immunoelectron microscopy experiments confirmed that AQP5 was to be found in the secretory granule membrane. Anti-AQP5 antibody evoked lysis of the secretory granules but anti-aquaporin-1 antibody did not and AQP1 was not detected in the secretory granule membranes by immunoblot analysis. When chloride ions were removed from the solution prepared for suspending secretory granules, the granule lysis induced by anti-AQP5 antibody was inhibited. Furthermore, 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid, an anion channel blocker, blocked the anti-AQP5 antibody-induced secretory granule lysis. These results suggest that AQP5 is, expressed in the parotid gland secretory granule membrane and is involved in osmoregulation in the secretory granules.

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Acknowledgments

We thank Dr. A. E. Hill, at University of Cambridge, for the critical reading of the manuscript and helpful discussions. This study was supported in part by Nihon University Multidisciplinary Research Grant for 2003–2004, Oral Health Science Center grant 5A06 from Tokyo Dental College, Grant-in Aid for 2003-Multidisciplinary Research Project from MEXT, and Grant-in Aid for Scientific Research from JSPS (#16390534, #14571751).

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Authors and Affiliations

  1. Department of Pathology and Oral Health Science Center, Tokyo Dental College, Mihama-ku, 261-8502, Chiba, Japan

    M. Matsuki, S. Hashimoto & M. Shimono

  2. Department of Physiology and Research Institute of Oral Science, Nihon University Scool of Dentistry at Matsudo, Matsudo, 271-8587, Chiba, Japan

    M. Matsuki, J. Fujita-Yoshigaki, S. Furuyama & H. Sugiya

  3. Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, 444-8585, Japan

    M. Murakami

Authors
  1. M. Matsuki
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  2. S. Hashimoto
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  3. M. Shimono
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  4. M. Murakami
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  5. J. Fujita-Yoshigaki
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  6. S. Furuyama
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  7. H. Sugiya
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Correspondence to H. Sugiya.

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Matsuki, M., Hashimoto, S., Shimono, M. et al. Involvement of Aquaporin-5 Water Channel in Osmoregulation in Parotid Secretory Granules. J Membrane Biol 203, 119–126 (2005). https://doi.org/10.1007/s00232-005-0736-9

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  • DOI: https://doi.org/10.1007/s00232-005-0736-9

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