Abstract
The marine dinoflagellate Cochlodinium polykrikoides is a harmful and highly motile algal species. To distinguish between the motility characteristics of solitary and chain-forming cells, the swimming trajectories and speeds of solitary cells and 2- to 8-cell chains of C. polykrikoides were measured using a digital holographic particle tracking velocimetry (PTV) technique. C. polykrikoides cells exhibited helical swimming trajectories similar to other dinoflagellate species. The swimming speed increased as the number of cells in the chain increased, from an average of 391 μm s−1 (solitary cells) to 856 μm s−1 (8-cell chain). The helix radius R and pitch P also increased as the number of cells in the chain increased. R increased from 9.24 μm (solitary cell) to 20.3 μm (8-cell chain) and P increased from 107 μm (solitary cell) to 164 μm (8-cell chain). The free thrust-generating motion of the transverse flagella and large drag reduction in the chain-forming cells seemed to increase the swimming speed compared to solitary cells. The measured swimming speeds agreed with those from field observations. The superior motility of chain-forming C. polykrikoides cells may be an important factor for its bloom, in addition to the factors reported previously.
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References
Baek SJ, Lee SJ (1996) A new two-frame particle tracking algorithm using match probability. Exp Fluids 22:23–32
Band-Schmidt CJ, Lilly EL, Anderson DM (2003) Identification of Alexandrium affine and A. margalefi (Dinophyceae) using DNA sequencing and LSU rDNAbased RFLP–PCR assays. Phycologia 42:261–268
Bauerfeind E, Elbrächter M, Steiner R, Throndsen J (1986) Application of laser doppler spectroscopy (LDS) in determining swimming velocities of motile phytoplankton. Mar Biol 93:323–327
Bolli L, Llaveria G, Garces C, Guadayol O, van Lenning K, Peters F, Berdalet E (2007) Modulation of ecdysal cyst and toxin dynamics of two Alexandrium (Dinophyceae) species under small-scale turbulence. Biogeosciences 4:559–567
Choi YS, Lee SJ (2009) Three-dimensional volumetric measurement of red blood cell motion using digital holographic microscopy. Appl Opt 48:2983–2990
Crenshaw H (1989) Kinematics of helical motion of microorganisms capable of motion with four degrees of freedom. Biophys J 56:1029–1035
Crenshaw H, Ciampaglio C, McHenry M (2000) Analysis of the three-dimensional trajectories of organisms: estimates of velocity, curvature and torsion from positional information. J Exp Biol 203:961–982
Fenchel T (2001) How Dinoflagellates swim. Protist 152:329–338
Fraga S, Gallager SM, Anderson DM (1989) Chain-forming dinoflagellates: an adaptation to red tides. In: Okaichi T, Anderson DM, Nemoto T (eds) Red tides: biology, environmental science, and toxicology. Elsevier, Amsterdam, pp 281–284
Gaines G, Taylor FJR (1985) Form and function of the dinoflagellate transverse flagellum. J Protozool 32:290–296
Goldstein SF (1992) Flagellar beat patterns. In: Melkonian M (ed) Algal cell motility. Chapman and Hall, New York, pp 99–153
Hand WG, Schmidt JA (1975) Phototactic orientation by the marine dinoflagellate Gyrodinium dorsum Kofoid. II. Flagellar activity and overall response mechanism. J Protozool 22(4):494–498
Hoppenrath M, Leander BS (2007) Morphology and phylogeny of the pseudocolonial dinoflagellates polykrikos lebourae and polykrikos herdmanae n. sp. Protist 158:209–227
Jiang H, Paffenhöfer G (2008) Hydrodynamic signal perception by the copepod Oithona plumifera. Mar Ecol Prog Ser 373:37–52
Kamykowski D, Reed RE, Kirkpatrick GJ (1992) Comparison of sinking velocity, swimming velocity, rotation and path characteristics among six marine dinoflagellate species. Mar Biol 113:319–328
Karleskint G Jr, Turner R, Small JW Jr (2006) Introduction to marine biology, 2nd ed. Thomson Brooks/Cole, Canada, pp 126–127
Karp-Boss L, Boss E, Jumars PA (2000) Motion of dinoflagellates in simple shear flow. Limnol Oceanogr 45:1594–1602
Kim HG et al (1997) Recent red tides in Korean coastal waters. Bull national fisheries research and development agency, pp 237–239
Kim CS, Lee SG, Lee CK, Kim HG, Jung J (1999) Reactive oxygen species as causative agents in the ichthyotoxicity of the red tide dinoflagellate Cochlodinium polykrikoides. J Plank Res 21:2105–2115
Kim C, Kim H, Kim C, Oh H (2007) Life cycle of the ichthyotoxic dinoflagellate Cochlodinium polykrikoides in Korean coastal waters. Harmful Algae 6:104–111
Kudela RM, Ryan JP, Blakely MD, Lane JQ, Peterson TD (2008) Linking the physiology and ecology of Cochlodinium to better understand harmful algal bloom events: a comparative approach. Harmful Algae 7:278–292
Lee YS (2006) Factors affecting outbreaks of high-density Cochlodinium polykrikoides red tides in the costal seawaters around Yeosu and Tongyeong, Korea. Mar Pollut Bull 52:1249–1259
Levandowsky M, Kaneta PJ (1987) Behavior in dinoflagellates. In: Taylor FJR (ed) The biology of dinoflagellates. Blackwell, Oxford, 360–397
Lewis NI, Xu W, Jericho SK, Kreuzer HJ, Jericho MH, Cembella AD (2006) Swimming speed of three species Alexandrium (dinophyceae) as determined by digital in-line holographiy. Phycologia 45:61–71
Matsuoka K, Iwataki M, Kawami H (2008) Morphology and taxonomy of chain-forming species of the genus Cochlodinium (Dinophyceae). Harmful Algae 7:261–270
Miyasaka I, Nanba K, Furuya K, Nimura Y (1998) High-speed video observation of swimming behavior and flagellar motility of Prorocentrum minimum (Dinophyceae). Protoplasma 204:38–46
Miyasaka I, Nanba K, Furuya K, Nimura Y, Azuma A (2004) Functional roles of the transverse and longitudinal flagella in the swimming motility of Prorocentrum minimum (Dinophyceae). J Exp Biol 207:3055–3066
Park JG, Jeong MK, Lee JA, Cho KJ, Kwon OS (2001) Diurnal vertical migration of a harmful dinoflagellate, Cochlodinium polykrikoides (Dinophyceae), during a red tide in coastal waters of Namhae island, Korea. Phycologia 40:292–297
Sheng J, Malkiel E, Katz J, Adolf J, Belas R, Place AR (2007) Digital holographic microscopy reveals prey-induced changes in swimming behavior of predatory dinoflagellates. Proc Natl Acad Sci USA 104(44):17512–17517
Smayda TJ (2002) Turbulence, watermass stratification, and harmful algal blooms: an alternative view and frontal zones as “Pelagic Seed Banks”. Harmful Algae 1:95–112
Smayda TJ (2010) Adaptations and selection of harmful and other dinoflagellate species in upwelling systems. 2. Motility and migratory behavior. Prog Oceanogr 85:71–91
Sullivan E, Swift E, Donaghay PL, Rines JEB (2003) Small-scale turbulence affects the division rate and morphology of two red-tide dinoflagellates. Harmful Algae 2:183–199
Tomas C, Smayda TJ (2008) Red tide blooms of Cochlodinium polykrikoides in a coastal cove. Harmful Algae 7:308–317
Vladimirov VA, Wu MSC, Pedley TJ, Denissenko PV, Zakhidova SG (2004) Measurement of cell velocity distributions in populations of motile algae. J Exp Biol 207:1203–1216
Vogel S (1994) Life in moving fluids; the physical biology of flow, 2nd ed. Princeton University, 337
Yu L, Kim MK (2005) Wavelength-scanning digital interference holography for tomographic three-dimensional imaging by use of the angular spectrum method. Opt Lett 3:2092–2094
Acknowledgments
This work was supported by the Creative Research Initiatives (Diagnosis of Biofluid Flow Phenomena and Biomimic Research) of the Ministry of Education, Science and Technology/National Research Foundation of Korea. This research was also supported by the World Class University program through the MEST/NRF of Korea (R31-2008-000-10105-0).
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Communicated by U. Sommer.
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Sohn, M.H., Seo, K.W., Choi, Y.S. et al. Determination of the swimming trajectory and speed of chain-forming dinoflagellate Cochlodinium polykrikoides with digital holographic particle tracking velocimetry. Mar Biol 158, 561–570 (2011). https://doi.org/10.1007/s00227-010-1581-7
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DOI: https://doi.org/10.1007/s00227-010-1581-7