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Localization of Pigment Epithelium-Derived Factor in Growing Mouse Bone

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Abstract

Pigment epithelium-derived factor (PEDF) is a potent anti-angiogenic factor found in a wide range of fetal and adult tissues, where it is thought to play a role in the regulation of angiogenesis during development. The temporal expression of PEDF during endochondral bone formation has not previously been reported. In this study, we analysed the expression pattern of PEDF in growing mouse hindlimbs from newborn day one through to maturation at week 9, using immunohistochemistry and in situ hybridization. PEDF expression was demonstrated in chondrocytes within the resting, proliferative and upper hypertrophic zones of the epiphyseal growth plate. The pattern of expression was consistent throughout the developmental stages of the mouse. In addition, PEDF was expressed by osteoblasts lining the bone spicules in the ossification zone of metaphyseal bone, as well as by osteoblasts lining cortical periosteum. These novel results demonstrate that PEDF is developmentally expressed in both cartilage and bone cells during endochondral bone formation, and strongly suggest that it may play a regulatory role in the processes of chondrocyte and osteoblast differentiation, endochondral ossification, and bone remodelling during growth and development of long bones.

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Acknowledgments

This study was supported by the Victorian Orthopaedic Research Trust, Australian Orthopaedic Association, and the Anti-Cancer Council of Victoria. The first author is a recipient of scholarships awarded by the Faculty of Medicine, University of Melbourne, and the National Health and Medical Research Council of Australia. The authors would like to thank Professor T. J. Martin for his helpful advice regarding the manuscript.

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Authors and Affiliations

  1. Department of Orthopaedics, University of Melbourne, St. Vincent’s Hospital Melbourne, Fitzroy, 3065, Australia

    Gerald M. Y. Quan, Joseline Ojaimi, Yaping Li & Peter F. M. Choong

  2. Department of Medicine, University of Melbourne, St. Vincent’s Hospital Melbourne, Fitzroy, 3065, Australia

    Vicky Kartsogiannis & Hong Zhou

  3. Division of Surgical Oncology, Peter MacCallum Cancer Institute, East Melbourne, 3002, Australia

    Peter F. M. Choong

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  1. Gerald M. Y. Quan
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  2. Joseline Ojaimi
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  3. Yaping Li
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  4. Vicky Kartsogiannis
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  5. Hong Zhou
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  6. Peter F. M. Choong
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Correspondence to Peter F. M. Choong.

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Quan, G.M.Y., Ojaimi, J., Li, Y. et al. Localization of Pigment Epithelium-Derived Factor in Growing Mouse Bone. Calcif Tissue Int 76, 146–153 (2005). https://doi.org/10.1007/s00223-004-0068-2

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