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Pentavalent antimony uptake pathway through erythrocyte membranes: molecular and atomic fluorescence approaches

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Abstract

Previous studies by our group have shown that Sb(V) is able to enter red blood cells in a dynamic process and is reduced to Sb(III) by glutathione. The present study aims to investigate a possible entry pathway for Sb(V) through the erythrocyte membrane. Applying fluorescence spectroscopy studies with Laurdan and diphenylhexatriene (DPH) probes, it was found that there was no interaction between Sb(V) and membrane lipids. By comparing the Sb(V) entry percentages through lipid vesicles and sealed erythrocyte membranes, it was found that Sb(V) required protein channels to pass through the membrane. The competitive inhibition results using HCO3 − and Cl− showed that the Sb(V) uptake rate through the membrane fell approximately 50–70 % until full inhibition was reached, which was possibly due to the inhibition of the anion exchanger 1 (AE1) channel. Finally, the fluorescence measurements with the 5-iodoacetamidofluorescein (5-IAF) probe showed that Sb(V) interacted with membrane protein SH groups during this process.

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Acknowledgments

The authors gratefully acknowledge financial support from FONDECYT (project 1150528).

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Authors and Affiliations

  1. Institute of Chemistry, Pontificia Universidad Católica de Valparaíso, Avenida Universidad #330, Campus Curauma, Valparaíso, Chile

    Camila Barrera, Silvana López, Luis Aguilar, Manuel Bravo & Waldo Quiroz

  2. Institute of Biology, Pontificia Universidad Católica de Valparaíso, Avenida Universidad #330, Curauma, Valparaíso, Chile

    Camila Barrera & Luis Mercado

Authors
  1. Camila Barrera
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  2. Silvana López
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  3. Luis Aguilar
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  4. Luis Mercado
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  5. Manuel Bravo
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  6. Waldo Quiroz
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Correspondence to Waldo Quiroz.

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The authors declare that they have no competing interests.

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Published in the topical collection featuring Young Investigators in Analytical and Bioanalytical Science with guest editors S. Daunert, A. Baeumner, S. Deo, J. Ruiz Encinar, and L. Zhang.

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Barrera, C., López, S., Aguilar, L. et al. Pentavalent antimony uptake pathway through erythrocyte membranes: molecular and atomic fluorescence approaches. Anal Bioanal Chem 408, 2937–2944 (2016). https://doi.org/10.1007/s00216-015-9188-y

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  • DOI: https://doi.org/10.1007/s00216-015-9188-y

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