Skip to main content
SpringerLink
Log in

Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing

  • Rapid Communication
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

We immobilized adherent human embryonic kidney (HEK) cells—which are able to trace adenosine triphosphate (ATP) —inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1a–e
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Eltzov E, Marks RS (2011) Anal Bioanal Chem 400:895–913

    Article  CAS  Google Scholar 

  2. Harms H, Wells MC, van der Meer JR (2006) App Microbiol Biotechnol 70:273–280

    Article  CAS  Google Scholar 

  3. Foudeh AM, Fatanat Didar T, Veres T, Tabrizian M (2012) Lab Chip 12:3249–3266

    Article  CAS  Google Scholar 

  4. Keusgen M (2002) Naturwissenschaften 89:433–444

    Article  CAS  Google Scholar 

  5. Raymond DE, Manz A, Widmer HM (1994) Anal Chem 66:2858–2865

    Article  CAS  Google Scholar 

  6. Kohlheyer D, Eijkel JCT, van den Berg A, Schasfoort RBM (2008) Electrophoresis 29:977–993

    Article  CAS  Google Scholar 

  7. Kostal V, Fonslow BR, Arriaga EA, Bowser MT (2009) Anal Chem 81:9267–9273

    Article  CAS  Google Scholar 

  8. Podszun S, Vulto P, Heinz H, Hakenberg S, Hermann C, Hankemeier T, Urban GA (2012) Lab Chip 12:451–457

    Article  CAS  Google Scholar 

  9. Köhler S, Nagl S, Fritzsche S, Belder D (2012) Lab Chip 12:458–463

    Article  Google Scholar 

  10. Becker M, Budich C, Deckert V, Janasek D (2009) Analyst 134:38–40

    Article  CAS  Google Scholar 

  11. Jezierski S, Belder D, Nagl S (2013) Chem Comm 49:904–906

    Article  CAS  Google Scholar 

  12. Oja SM, Wood M, Zhang B (2013) Anal Chem 85:473–486

    Article  CAS  Google Scholar 

  13. Steude A, Schmidt S, Robitzki AA, Pänke O (2011) Lab Chip 11:2884–2892

    Article  CAS  Google Scholar 

  14. Richter L, Stepper C, Mak A, Reinthaler A, Heer R, Kast M, Brückl H, Ertl P (2007) Lab Chip 7:1723–1731

    Article  CAS  Google Scholar 

  15. Asphahani F, Zhang M (2007) Analyst 132:835–841

    Article  CAS  Google Scholar 

  16. Köhler S, Benz C, Becker H, Beckert E, Beushausen V, Belder D (2012) RSC Adv 1:520–525

    Article  Google Scholar 

  17. Schneider CA, Rasband WS, Eliceiri KW (2012) Nat Methods 9:671–675

    Article  CAS  Google Scholar 

  18. Graham FL, Smiley J, Russel WC, Nairn R (1977) J Gen Virol 36:59–72

    Article  CAS  Google Scholar 

  19. Fischer W, Franke H, Groger-Arndt H, Illes P (2005) Naunyn-Schmiedeberg’s Arch Pharmacol 371:466–472

    Article  CAS  Google Scholar 

  20. Bondy B, Bauer J, Seuffert I, Weber G (1995) Electrophoresis 16:92–97

    Article  CAS  Google Scholar 

  21. Wang F, Wang H, Wang J, Wang H-Y, Rummel PL, Garimella SV, Chang L (2008) Biotechnol Bioeng 100:150–158

    Article  CAS  Google Scholar 

  22. North RA (2002) Physiol Rev 82:1013–1067

    CAS  Google Scholar 

  23. Guerini D, Coletto L, Carafoli E (2005) Cell Calcium 38:281–289

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Analytische Chemie, Universität Leipzig, Johannisallee 29, 04103, Leipzig, Germany

    Stefan Jezierski, Christian Benz, Stefan Nagl & Detlev Belder

  2. Rudolf-Boehm-Institut für Pharmakologie und Toxikologie, Universität Leipzig, Härtelstr. 16-18, 04107, Leipzig, Germany

    Anke S. Klein & Michael Schaefer

Authors
  1. Stefan Jezierski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anke S. Klein
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christian Benz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael Schaefer
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Stefan Nagl
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Detlev Belder
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Detlev Belder.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 934 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jezierski, S., Klein, A.S., Benz, C. et al. Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing. Anal Bioanal Chem 405, 5381–5386 (2013). https://doi.org/10.1007/s00216-013-6945-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-013-6945-7

Keywords

Search

Navigation