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“Do it yourself” reference materials for δ 13C determinations by isotope ratio mass spectrometry

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Abstract

A method is described to prepare inexpensive organic reference materials for routine stable isotope work. The method blends isotope-enriched and isotope-depleted materials to match closely international measurement standards. Examples are presented showing how 13C-enriched and 13C-depleted glucose were blended to match the isotopic compositions of the international carbon isotope standards, NBS 19 and L-SVEC. Preparation of the blended standards was straightforward, and the prepared solutions proved easy to dispense into tin capsules and easy to combust in a conventional isotope analysis system. The blended materials allow a laboratory to perform a two-point calibration for isotopic composition on a daily basis, with the use of an international reference material recommended for routine quality assurance. The blending method can be applied widely to make isotope standards for other elements and compounds.

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Notes

  1. It is also important that RMs are easy to handle, non-hygroscopic and chemically similar to routine samples.

  2. Close bracketing may be appropriate if memory effects in the sample preparation lines are substantial.

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Authors and Affiliations

  1. Queensland Health Forensic and Scientific Service, Archerfield, PO Box 594, Brisbane, QLD, 4109, Australia

    J. F. Carter

  2. Griffith University - Australian Rivers Institute, Kessels Road, Nathan, Brisbane, QLD, 4111, Australia

    B. Fry

Authors
  1. J. F. Carter
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  2. B. Fry
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Correspondence to J. F. Carter.

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Carter, J.F., Fry, B. “Do it yourself” reference materials for δ 13C determinations by isotope ratio mass spectrometry. Anal Bioanal Chem 405, 4959–4962 (2013). https://doi.org/10.1007/s00216-013-6851-z

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  • DOI: https://doi.org/10.1007/s00216-013-6851-z

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