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Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization

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Abstract

We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor array, which will make it highly versatile for various research purposes.

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Acknowledgments

The Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract No. DE-AC02-07CH11358. This work was supported by the Director of Science, Office of Basic Energy Science, Division of Chemical Sciences.

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Authors and Affiliations

  1. Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA, 50011, USA

    Changbei Ma, Edward S. Yeung, Shengda Qi & Rui Han

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  1. Changbei Ma
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  2. Edward S. Yeung
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  3. Shengda Qi
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  4. Rui Han
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Correspondence to Edward S. Yeung.

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Ma, C., Yeung, E.S., Qi, S. et al. Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization. Anal Bioanal Chem 402, 2217–2220 (2012). https://doi.org/10.1007/s00216-011-5653-4

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  • DOI: https://doi.org/10.1007/s00216-011-5653-4

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