Abstract
A new micellar electrokinetic chromatography (MEKC) method using beta-cyclodextrins (β-CDs) and 1-butyl-3-methylimidazolium hexafluorophosphates (ionic liquids) as additives was successfully developed for determination of para-, meta-, and ortho-phenylenediamines isomers (p-P, m-P, and o-P) in hair dyes. To improve the sensitivity of the MEKC-UV, a simple and cheap flow injection (FI) technique using a micro-column packed with coal cinders (the by-products from combustion in a boiler) as solid-phase extractant was also investigated. In the presence of 20 mmol L−1 phosphates at pH 5.5, addition of 12 mmol L−1 ionic liquids and 8 mmol L−1 β-CDs greatly improved the separation efficiency. The three analytes could be quantitatively adsorbed by coal cinders, and desorbed readily with 0.15 mL of 0.01 mol L−1 NaOH. Under the optimum conditions, an enrichment factor (EF) of 33.3 was obtained, and determination limits of p-P, m-P, and o-P were 1.97 × 10−7, 0.99 × 10−7, and 0.61 × 10−7 mol L−1, respectively. The adsorption capacities of the coal cinders micro-column for p-P, m-P, and o-P were all 1.20 mg g−1. The presented procedure was successfully applied to the determination of p-P, m-P, and o-P in hair dyes with satisfactory results.
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Acknowledgements
This project was financially supported by National Nature Science Foundation of China (No. 20975031, 21075030), Education Committee of Hubei Province (Q20102506), Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education (ACBM2010004), Young and Middle-Aged Elitists’ Scientific and Technological Innovation Team Project of Hubei Education Department of China under Grant (T201007).
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Wu, Y., Jiang, F., Chen, L. et al. Determination of phenylenediamine isomers in hair dyes by coal cinders micro-column extraction and MEKC. Anal Bioanal Chem 400, 2141–2147 (2011). https://doi.org/10.1007/s00216-011-4932-4
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DOI: https://doi.org/10.1007/s00216-011-4932-4